Bupirimate-containing antibacterial composition
A pyrithrimol sulfonate and composition technology, which is applied in the direction of fungicides, biocides, biocides, etc., to achieve good killing effect, easy degradation, and good drug effect
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example 1
[0045] (1) Experimental object:
[0046] Cucumber powdery mildew pathogen was isolated and purified from cucumber powdery mildew-infected leaves in Shandong Shouguang cucumber greenhouse, cultured and preserved on PDA slant in 4°C refrigerator.
[0047] Test medium:
[0048] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
[0049] AEA medium: yeast powder 5g / L, glycerol 20mL / L, MgSO 4 0.25g / L, NaNO 3 6g / L, KCl 0.5g / L, KH 2 PO 4 1.5g / L, agar powder 20g / L, deionized water 1L.
[0050] (2) Experimental medicine:
[0051] 95% Propoxyquinoline original drug, 95% Pyrimethymol sulfonate original drug, provided by Synthetic Institute of Qingdao Hailier R&D Center.
[0052] Mix and match settings:
[0053] The content percentages of 18% pyrithrimolsulfonate·propoxyquinoline are 3:15, 5:13, 7:11, 9:9, 11:7, that is, 3%+15%, 5%+13%, 7%+11%, 9%+9%, 11%+7% total 5 mixing combinations.
[0054] (3) Test method:
[0055] Toxicity determination of single agent against...
example 2
[0073] Biological Assay Example 2: Indoor Toxicity Test of Phenoxyquinoline and Pyrimethol Sulfonate on Wheat Powdery Mildew.
[0074] Wheat powdery mildew pathogen is isolated and purified from wheat powdery mildew diseased leaves in the field, cultivated and stored in a 4°C refrigerator on a PDA slope.
[0075] We performed toxicity assays for single doses of pyrithrimol sulfonate and phenoxyquinoline, EC 50 The values were 4.53 mg / l and 7.76 mg / l, respectively.
[0076] Mix and match settings:
[0077] The content percentages of 20% pyrithrimolsulfonate·phenoxyquinoline are 4:16, 6:14, 8:12, 10:10, 12:8, namely 4%+16%, 6%+14%, 8%+12%, 10%+10%, 12%+8% total 5 mixing combinations.
[0078] The test method is similar to that of biological assay example 1.
[0079] Experimental results and analysis:
[0080] Table 3 Toxicity test of phenoxyquinoline and pyrithrimol sulfonate compound against wheat powdery mildew
Virulence regression...
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