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Penicillium capable of resisting false smut of rice and application thereof

A technology of rice smut and penicillium, applied in the direction of application, fungicides, fungi, etc., to achieve the effects of reducing pollution, strong inhibitory activity, and effective control

Active Publication Date: 2012-02-08
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, few such preparations are commercially produced, and their application needs further research

Method used

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  • Penicillium capable of resisting false smut of rice and application thereof
  • Penicillium capable of resisting false smut of rice and application thereof
  • Penicillium capable of resisting false smut of rice and application thereof

Examples

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Embodiment 1

[0040] A kind of separation and screening of penicillium with antagonistic effect on rice smut bacterium, its steps are as follows:

[0041] a. Grind the soil sample finely, place about 5 grams of the sample in a sterile petri dish, pour it into a PDA medium at 33 or 34 or 35 or 36 or 37°C, and culture it at 28°C for 7 days to isolate a single fungal colony. The composition of the PDA medium is: 20% potato juice (weight / volume), 2% glucose (weight / volume), pH 6.0. The isolated fungal strains were inoculated in PS (potato sucrose medium), 28°C, 180rpm, constant temperature shaking culture for 96h. Collect the fermentation broth, remove mycelium by filtering with filter paper, and centrifuge at 10,000 rpm at 4°C for 20 minutes; collect and centrifuge the supernatant through a 0.22 μm filter membrane to obtain sterile supernatant, and store it at 4°C until use. The composition of the PS medium is: 20% potato juice (weight / volume), 2% sucrose (weight / volume), pH 6.0.

[0042] b....

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Abstract

The invention discloses penicillium capable of resisting false smut of rice and an application thereof. The penicillium is prepared through the following steps of: A, ustilaginoidea virens isolation, wherein a ustilaginoidea virens ear is collected, and the ustilaginoidea virens is isolated by adopting a chlamydospore suspension method; B, antibacterial isolation and culture of the ustilaginoideavirens, wherein a soil sample is ground, placed in an aseptic petri dish, and poured into a PDA (potato dextrose agar) culture medium to culture and isolate a single colony of fungus, the isolated fungus strain is inoculated into a PSA (prostate-specific antigen) culture solution for shaking culture, and the fermentation broth is filtered and degermed to obtain an aseptic supernatant sample; and C, antagonist screening of the ustilaginoidea virens, wherein a conidiophore suspension of the ustilaginoidea virens is coated on a PSA panel, an aseptic Oxford plate is placed in the panel, and the aseptic supernatant sample is added into the aseptic Oxford plate to culture penicillium. The invention also provides an application of the antagonist penicillium AF-25 in preparing a biological pesticide for controlling or preventing the false smut of rice. The penicillium has an antagonistic effect on ustilaginoidea virens, and can inhibit spore germination and mycelial growth of the ustilaginoidea virens. The fermentation broth of the penicillium has strong inhibition activity to the false smut.

Description

technical field [0001] The present invention relates to the microbial control of rice smut fungus [Ustilaginoidea virens (Cke) Tak], more specifically relates to a strain of Penicillium chrysogenum which has antagonistic effect on rice smut fungus (Penicillium chrysogenum), and also relates to the preparation method of the bacterium , also relates to the purposes of this bacterium. The strain of Penicillium is a fungus, which can inhibit the germination of rice smut spores and mycelia growth, and its antibacterial active substances are mainly small molecule polypeptides with a molecular weight less than 3500Da or derivatives thereof. The fermentation liquid of the strain has strong inhibitory activity against rice smut bacteria, and has good storage stability, acid-base and temperature tolerance. Animal toxicity experiments show that the fermentation supernatant of the strain has no effect on experimental rats. It is obviously toxic and belongs to the level of slight toxicity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/80
Inventor 高梅影刘标刘朋明吴燕
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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