Method for separating hemopoietic stem cells through cord blood separation double bag
A technology of hematopoietic stem cells and umbilical cord blood, which is applied in the field of separating stem cells, can solve problems such as being easily contaminated, and achieve the effects of saving operation time, high safety performance, and safe and effective concentration
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Embodiment 1
[0030] A method for separating hematopoietic stem cells with two bags of umbilical cord blood separation, comprising a 200ml main bag 1 and a 150ml transfer bag 2, the main bag is connected with a feeding tube 7 and a transfer connecting tube 3, and the feeding tube is provided with a feeding control The valve 10 and the 0.22um filter 11 are connected with the parallel inlet blood vessel 6 and the transfer tube 5 on the transfer tube, the blood inlet control valve 9 and the blood filter 13 are arranged on the inlet blood vessel, and the transfer tube 5 is connected with the transfer bag 2, and The transfer pipe 5 is also provided with a transfer control valve 8, the transfer connecting pipe 3 is also provided with a sampling port 12, and the main bag is also provided with a cell outlet 4 externally connected to a cell freezing bag;
[0031] The method of isolating hematopoietic stem cells comprises the steps of:
[0032] (a) After mixing the blood in the umbilical cord blood c...
Embodiment 2
[0045] The method of isolating hematopoietic stem cells comprises the steps of:
[0046] (a) After mixing the blood in the umbilical cord blood collection bag, centrifuge at a speed of 2000 rpm for 30 minutes;
[0047] (b) Transfer the upper layer of blood to the main bag through the inlet blood vessel with the blood filter through the blood separation clamp, and the remaining 20ml of red blood cells will not be transferred and discarded;
[0048] (c) Add 1 / 5 volume of hydroxyethyl starch HES from the feeding tube with a 0.22um filter to the main bag, and close the transfer control valve after transfer;
[0049] (d) Mix the blood components of the main bag with hydroxyethyl starch HES, put the cord blood separation double bag into a centrifuge as a whole, and centrifuge at 1500 rpm for 15 minutes with the main bag interface facing up;
[0050] (e) Open the transfer control valve, and transfer the 2 / 3 plasma that was placed on the upper layer of the main bag after centrifugation...
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