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Bi-path detecting card capable of simultaneously detecting carbamazepine and sodium valproate and detecting method of same

A technology of carbamazepine and sodium valproate, which is applied in the field of blood drug concentration detection of antiepileptic drugs, to achieve the effects of saving detection costs, easy preparation, and rapid detection

Inactive Publication Date: 2012-01-25
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of utensil that can detect carbamazepine and sodium valproate simultaneously, overcome the defect that prior art operation is loaded down with trivial details, professional requirement is high, Used to detect blood drug concentration to ensure drug safety

Method used

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  • Bi-path detecting card capable of simultaneously detecting carbamazepine and sodium valproate and detecting method of same
  • Bi-path detecting card capable of simultaneously detecting carbamazepine and sodium valproate and detecting method of same
  • Bi-path detecting card capable of simultaneously detecting carbamazepine and sodium valproate and detecting method of same

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Experimental program
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Effect test

Embodiment 1

[0024] A kind of dual-channel detection card of carbamazepine and sodium valproate, it is to arrange test strip 2 in detection card shell 1, the structure of detection card shell 1 and the setting of test strip 2 refer to appendix figure 1 And attached image 3 , The detection card shell 1 is a long flat shell-shaped shell with a length of 7cm, a width of 2cm, a thickness of 0.5cm, and a wall thickness of the thin shell of 0.1cm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0025] The test strip 2 is a thin strip with a multilayer structure, the length of the strip is about 6.0cm, the width of the strip is about 0.4cm, and the thickness is less than 2.5mm. The test strip 2 has a multilayer structure: the bot...

Embodiment 2

[0028] The sample processing method is:

[0029] After the patient took the medicine continuously for one week, draw 2mL of venous blood before taking the medicine in the morning, centrifuge at 3000r / min for 10min, separate the serum, process it immediately or store it within 48h at -20°C;

[0030] The main reaction of the detection card is the immunological antigen and antibody reaction. The colloidal gold-labeled antibody that migrates on the nitrocellulose membrane is coupled with the protein conjugate containing carbamazepine and sodium valproate on the test strip. Objects, and quality control with anti-mouse antibody reaction, forming a brown-red band. If the corresponding carbamazepine and sodium valproate to be detected in the sample are all higher than the allowable value, they will react with the corresponding antibody after the sample is added, and will not couple with the carbamazepine protein contained in the corresponding detection band. Conjugates, sodium valpro...

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Abstract

The invention provides a bi-path detecting card capable of simultaneously detecting carbamazepine and sodium valproate and a detecting method of the same, and belongs to the technical field of the monitoring on the blood concentration of antiepileptic drug. In the invention, a detecting window and a sample adding hole are arranged on the surface of a shell of the detecting card; a testing bar is arranged in the shell; a nitrocellulose film is adhered to the middle part of a backing board of the testing bar; a water absorbent film is adhered to one end of the backing board; a sample pad is adhered to the other end of the backing board; two segments of colloidal gold films are arranged between the water absorbent film and the sample pad in an adhesive manner; the colloidal gold films are respectively glass fiber films containing colloidal gold markers of monoclonal antibodies resistant to the carbamazepine and the sodium valproate; three separated imprint display strips are transversely arranged on the nitrocellulose film, and include a detecting strip containing carbamazepine protein conjugate, an another detecting strip containing sodium valproate protein conjugate and a quality control strip containing antibodies resistant to a rabbit or a rat; the sample pad is just opposite to the sample adding hole; and the nitrocellulose film is just opposite to the detecting window. The detecting card can be used for simultaneously detecting components of two anticonvulsants in a serum sample, and has the advantages of saved cost for detection, convenience for use, quickness in detection, high sensitivity and accuracy in result.

Description

technical field [0001] The invention relates to a device for detecting blood drug concentration of antiepileptic drugs, in particular to a dual-channel detection card for simultaneously detecting carbamazepine and sodium valproate, belonging to the technical field of detection of blood drug concentration of antiepileptic drugs. Background technique [0002] Carbamazepine (CBZ) is an iminostilbene derivative with a chemical structure similar to that of the tricyclic antidepressant imiprazole. In 1962, Bolm was first used to treat trigeminal neuralgia, and the curative effect was satisfactory, and then it was used to treat epilepsy and other diseases. Its antiepileptic effect is similar to that of phenytoin sodium, which can enhance the stability of cell membrane potential and prevent the abnormal potential activity of the brain from spreading to the surrounding brain tissue, thus preventing the onset of epilepsy. It is a broad-spectrum antiepileptic drug, widely used in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
Inventor 周坚吴文晔熊晓芳
Owner 无锡安迪生物工程有限公司
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