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Microbial fermentation enzymolysis method for extracting grease and protein of soybean

A technology of microbial fermentation and soybean oil, which is applied in the extraction of soybean oil and protein, and in the field of soybean oil and protein extraction through microbial fermentation liquid enzymolysis, which can solve the problem of increasing the complexity of the process, not getting rid of the use of organic solvents, and the actual yield is low problems such as reducing the workload of water washing to remove salt, reducing the workload of process operations, and reducing the use of alkali

Inactive Publication Date: 2013-11-27
NORTHEAST AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods have the following advantages and disadvantages: the advantage of the solvent leaching method is that the oil yield is higher, but the solvent in this method is easy to remain, which is harmful to the human body, and the protein denaturation is serious during the high temperature desolventization process of the wet meal. The use of organic solvents increases the complexity of the process, large investment in equipment, high energy consumption, poor production safety, and pollution to the environment.
The raw materials of this process are not pretreated, and they undergo two enzymatic hydrolysis during the oil extraction process. Due to the limitation of biological enzyme technology at that time, only alkaline protease and neutral protease were used for hydrolysis. Not only the process is complicated, but also the oil extraction rate Can only reach about 65%
In 2000, Professor He Guoqing of Zhejiang University studied the extraction of soybean oil with aqueous enzyme organic solvents, and obtained the best enzyme selection, the best enzymatic hydrolysis process conditions and extraction conditions, and the extraction rate of soybean oil reached about 84%, but due to the technology at that time limitations, these methods do not get rid of the use of organic solvents
[0011] Existing aqueous enzymatic oil extraction technologies all have the problems of high difficulty in demulsification process and low yield of free oil to varying degrees, which directly affects the actual recovery yield of oil in soybeans (the lower the demulsification rate, the higher the yield of oil in soybeans). The lower the actual yield), thus indirectly increasing the cost

Method used

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  • Microbial fermentation enzymolysis method for extracting grease and protein of soybean
  • Microbial fermentation enzymolysis method for extracting grease and protein of soybean
  • Microbial fermentation enzymolysis method for extracting grease and protein of soybean

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0053] Experimental example 1 Composition of liquid fermentation medium and optimization experiment of fermentation conditions

[0054] 1 Materials and methods

[0055] 1.1 Experimental materials

[0056] 1.1.1 Strains

[0057] Bacillus subtilis was purchased from China Industrial Microorganism Culture Collection Center (ATCC20524).

[0058] 1.1.2 Reagents

[0059] Beef extract, peptone, glucose, soluble starch, maltose, sucrose, tyrosine, folin phenol reagent, trichloroacetic acid, anhydrous sodium carbonate, and sodium chloride are all analytically pure; Tris-Hcl buffer solution (pH8. 9 is suitable for the determination of alkaline protease activity, pH 7.2 is suitable for the determination of neutral protease activity).

[0060] 1.1.3 Raw materials

[0061] Low-temperature defatted soybean meal: purchased from Harbin Gaoke Biotechnology Company.

[0062] 1.1.4 Medium

[0063] Incline preservation medium: 1% peptone, 0.5% beef extract, 0.5% sodium chloride, 1.5% agar,...

experiment example 2

[0144] Experimental example 2 Process optimization experiment of enzymatic hydrolysis conditions and demulsification conditions

[0145] 1 Materials and methods

[0146] 1.1 Experimental materials

[0147] Raw materials: extruded soybeans.

[0148] 1.2 Extrusion and expansion: the moisture content of the material is 13%-17wt%, the sleeve temperature of the extrusion extruder is 85-110°C, and the screw speed is 95-115r / min.

[0149] 1.3 Crush: Crush the extruded material and pass it through a 70-120 mesh sieve.

[0150] 1.4 Experimental method

[0151] 1.4.1 Process flow

[0152] Such as Figure 19 shown.

[0153] 1.4.2 Test method:

[0154] Determination of protein content: Kjeldahl method

[0155] Determination of fat content in milk: Gothic-Ross method

[0156] Oil content in residue: Soxhlet extraction

[0157] 1.4.3 Test formula

[0158]

[0159]

[0160]

[0161]

[0162]

[0163]

[0164] 1.4.4 Determination of the process conditions for extr...

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Abstract

The invention discloses a microbial fermentation enzymolysis method for extracting the grease and protein of soybean, comprising the following steps of: (1) carrying out pretreatment on the soybean; (2) inoculating microbial strains into liquid fermentation culture medium for fermentation and enzyme production; (3) carrying out eccentricity on the fermentation culture medium, and mixing the supernatant liquor with water, and inoculating bean flour for enzymolysis; (4) centrifugating to obtain emulsible liquid, hydrolyzate and residue; and carrying out emulsion breaking and obtaining free oil; and (5) adjusting the pH value of the hydrolyzate, centrifugating, cleaning the precipitate, then centrifugating again, then adjusting the pH value after the precipitate is dissolved by water, drying and obtaining the isolated soy protein. In the microbial fermentation enzymolysis method, all process parameters of the oil and the protein of the soybean by microbial fermentation enzymolysis are studied comprehensively and systematically, the extraction rate of the total oil and the total protein combined with the free oil are adopted as the surveying index on the basis of determining the best culture medium and the culture conditions for fermentation and enzyme production, and the conditions for enzymolysis of soybean flour and emulsion breaking are determined, so that the extraction rate of the total oil in the raw material is up to more than 90%, and the yield of the total protein is up to more than 90%.

Description

technical field [0001] The invention relates to a method for extracting soybean oil and protein, in particular to a method for simultaneously extracting oil and protein from soybeans subjected to extrusion and puffing pretreatment through enzymolysis of microbial fermentation liquid, belonging to the field of soybean oil extraction. Background technique [0002] At present, there are two kinds of soybean oil production processes, one is solvent leaching method, and the other is mechanical pressing method. These two methods have the following advantages and disadvantages: the advantage of the solvent leaching method is that the oil yield is higher, but the solvent in this method is easy to remain, which is harmful to the human body, and the protein denaturation is serious during the high temperature desolventization process of the wet meal. The use of organic solvents increases the complexity of the process, resulting in large investment in equipment, high energy consumption,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C11B1/00C11B1/04C12R1/125
Inventor 江连洲吴海波李扬赵英程建军朱秀清孙树坤姚刚张平吴海涛张清春黄莉胡少新刑常瑞
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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