Riemerella anatipestifer antibody indirect ELISA method detection kit and application thereof
A technology of Duck Reemer's and detection kits, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as drug resistance, complex serotypes, threats to drug and food safety, etc., and achieve high specificity and Sensitivity, ease of large-scale promotion and application, and the effect of reducing subjectivity
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[0036] The present invention will be described in further detail below in conjunction with the examples, but this description will not constitute a limitation to the present invention.
[0037]An indirect ELISA detection kit for Riemerella anatipestifer antibodies, the kit includes: a) antibody detection plate, b) enzyme conjugate working solution, c) positive control, d) negative control, e) sample diluent , f) 10× concentrated washing solution, g) substrate chromogenic solution A, h) substrate chromogenic solution B and i) stop solution, wherein: the antibody detection plate is coated with Riemerella anatipestifer Detachable 96-well polystyrene ELISA plate for membrane protein (ompA), the enzyme conjugate working solution is horseradish peroxidase-labeled rabbit anti-duck IgY polyclonal antibody, and the positive control is immunization with the standard strain of Riemerella anatipestifer The hyperimmune serum was prepared from ducklings, and the negative control was the ne...
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