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Preparation method of donkey-hide gelatin oligopeptide

A technology of oligopeptides and donkey-hide gelatin, which is applied in the field of biocomposite enzymes to hydrolyze donkey-hide gelatin peptides. It can solve the problems of high cost, salty taste, large molecular weight, and non-concentrated molecular weight distribution, and achieve low cost, no toxic side effects, and concentrated molecular weight. Effect

Active Publication Date: 2015-01-14
JIANGZHONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention aims at the disadvantages of the enzymatic hydrolyzate in the prior art, such as bitterness, salty taste, large molecular weight, non-concentrated molecular weight distribution, and high cost, to provide a method for extracting donkey-hide gelatin oligopeptides, using donkey-hide gelatin as raw material with alkaline protease After enzymolysis, neutral protease-assisted secondary enzymolysis and flavor protease are used to exocut and adjust the flavor, and the ultrafiltration membrane with a molecular weight cut-off of 0.5-150,000 Daltons is used for ultrafiltration and membrane concentration and refining technology and spray drying to obtain The donkey-hide gelatin oligopeptide product; this method is easy to operate, has a good taste, low cost, environmental protection and high efficiency, and is very suitable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 20kg of donkey-hide donkey-hide gelatin crushed and passed through a 80-mesh sieve, add 160kg of distilled water, stir evenly, heat up to 85°C to inactivate lipase for 30min; cool to 60°C, add 400g of alkaline protease, stir for 2.5h Afterwards, add 200g neutral protease, 100g flavor protease, stir enzymolysis at 55°C for 3h, then heat up to 100°C, inactivate the enzyme for 15min; cool to room temperature, centrifuge at 4000r / min for 20min, collect supernatant, and pass Roll-type membrane ultrafiltration with a molecular weight cut-off of 30,000 Daltons, collecting the ultrafiltrate, concentrating the ultrafiltrate through a membrane, and spray-drying the concentrated solution to obtain 10.82kg of powder (light yellow).

[0020] Determination of donkey-hide gelatin oligopeptide content

[0021] 1. method summary

[0022] Low molecular weight protein hydrolyzate (including peptides and free amino acids) is soluble in trichloroacetic acid solution; high molecular ...

Embodiment 2

[0062] Weigh 30kg of donkey-hide donkey-hide gelatin that has been crushed and passed through a 60-mesh sieve, add 240kg of distilled water, stir evenly, heat up to 100°C to inactivate lipase for 60min; cool to 60°C, add 300g of alkaline protease, and stir for 4 hours to enzymatically hydrolyze , add 150g neutral protease, 75g flavored protease, stir at 70°C for 3h, then heat up to 100°C, inactivate the enzyme for 15min; cool to room temperature, centrifuge at 4000r / min for 20min, collect the supernatant, which is passed through a hollow Ultrafiltration membrane ultrafiltration, the molecular weight cut-off of the membrane is 150,000 Daltons, the ultrafiltrate is collected, the ultrafiltrate is concentrated through the membrane, and the concentrated solution is spray-dried to obtain 15.95 kg of powder.

Embodiment 3

[0064] Weigh 10kg of donkey-hide donkey-hide gelatin crushed and passed through a 100-mesh sieve, add 120kg of distilled water, stir evenly, heat up to 100°C to inactivate lipase for 60min; cool to 40°C, add 300g of alkaline protease, and stir for 4h to enzymatically hydrolyze , add 150g neutral protease, 25g flavored protease, stir enzymolysis at 40°C for 3h, then heat up to 100°C, inactivate enzyme for 15min; cool to room temperature, centrifuge at 4000r / min for 20min, collect the supernatant, and pass through Membrane ultrafiltration, the molecular weight cut-off of the membrane is 5,000 Daltons, the ultrafiltrate is collected, the ultrafiltrate is concentrated by the membrane, and the concentrated solution is spray-dried to obtain 6.16kg of powder.

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Abstract

The invention aims to provide an extraction method of donkey-hide gelatin oligopeptide. The method comprises the steps of: conducting enzymolysis to the raw material donkey-hide gelatin with an alkali protease, then adopting a neutral protease for assisting a secondary enzymolysis and a flavorzyme for circumscribing and flavor adjusting, conducting ultrafiltration with an ultrafilter membrane with a molecular weight cutoff of 5000-150000 Dalton, and carrying out membrane concentration and refining as well as spray drying, thus obtaining a donkey-hide gelatin oligopeptide product. Characterized by simple and convenient operation, good mouthfeel of product, low cost, environmental protection and high efficiency, the method provided in the invention is very suitable for large scale industrial production.

Description

technical field [0001] The invention relates to the technical field of deep processing of donkey-hide gelatin, in particular to the method of using biological compound enzymes to enzymolyze donkey-hide gelatin peptides [0002] Preparation. Background technique [0003] Ejiao mainly contains collagen (80%), and it is generally taken orally, while the protein in food or medicine is usually enzymatically hydrolyzed into oligopeptides or amino acids by the digestive juice to be absorbed and utilized by the body, and the above indications are common Weak spleen and stomach or gastrointestinal reactions, so the activity of digestive tract enzymes is low, and the digestion ability is poor. Therefore, oral administration of donkey-hide gelatin will not only cause a burden on the patient's digestive system, but also fail to achieve the desired drug effect. [0004] At present, the methods for extracting donkey-hide gelatin oligopeptides mainly include lye extraction and biological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 钟虹光易敏之卢建中刘根云马莉
Owner JIANGZHONG PHARMA
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