Semicontinuous fermentation technology of gluconate
A technology of gluconate and fermentation process, applied in the field of gluconate semi-continuous fermentation process, can solve the problems of low conversion rate, many by-products, difficult to control conditions, etc., and achieve the effect of reducing the number of times and saving sugar consumption
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Embodiment 1
[0010] The first step, seed culture: Add 2.5Kg of glucose, 75g of corn steep liquor, 6.4g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L culture tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.
[0011] The second step, fermentation: add 8.4Kg of glucose, 5.6g of magnesium sulfate, 0.56g of potassium dihydrogen phosphate, 2.8g of urea, and 2ml of defoamer to a 50L fermenter. At 38°C, inoculate the cultured seed solution with a 10% inoculation amount. Control the dissolved oxygen (DO) ≥ 30, the temperature is 38°C, adjust the pH to 5.5 with 20% calcium carbonate, start the fermentation, when the dissolved oxygen (DO) rises to 100, the fermentation ends, the fermentation time is 18-22 hours, the fermentation ends The hourly volume is 34-36L.
[0012] The third step, semi-continuous fermentation:
[0013] (1) Ingredients: Add 7Kg of glucose, 18g of...
Embodiment 2
[0022] The first step, seed culture: Add 2.5Kg of glucose, 75g of corn steep liquor, 6.4g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L culture tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.
[0023] The second step, fermentation: add 8.4Kg of glucose, 5.6g of magnesium sulfate, 0.56g of potassium dihydrogen phosphate, 2.8g of urea, and 2ml of defoamer to a 50L fermenter. At 38°C, inoculate the cultured seed solution with a 10% inoculation amount. Control the dissolved oxygen (DO) ≥ 30, the temperature is 38°C, adjust the pH to 5.5 with 32% sodium hydroxide, and start the fermentation. When the dissolved oxygen (DO) rises to 100, the fermentation ends. The fermentation time is 18-22 hours, and the fermentation ends The hourly volume is 34-36L.
[0024] The third step, semi-continuous fermentation:
[0025] (1) Ingredients: Add 7Kg of glucose,...
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