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Method and device for preparing gallic acid

A technology of gallic acid and tannic acid, which is applied in biochemical equipment and methods, methods for supporting/immobilizing microorganisms, and biochemical cleaning devices, etc., can solve the problems of many side reactions, darkening of crude product color, and long production cycle, etc. Achieve the effect of adapting to large-scale production, reducing production costs and shortening production cycle

Inactive Publication Date: 2012-01-18
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have the following problems: (1) the concentrated sulfuric acid used in the acid hydrolysis method has strong oxidizing properties, which can destroy the benzene ring structure in tannic acid, and there are many side reactions, which cause the gallic acid yield to decrease, and the crude product color becomes dark; ( 2) The alkaline hydrolysis method needs to neutralize the caustic soda with hydrochloric acid, so that the hydrolyzate contains a large amount of sodium chloride, the subsequent separation process is complicated, and the cost increases; (3) The hydrolysis of tannic acid is not complete during the hydrolysis process, the product quality is unstable, and the equipment is corroded Seriously, the acid-base wastewater produced seriously pollutes the environment
In this method, the temperature of fermentation enzyme production process and hydrolysis process is low, the production cycle is long, the hydrolysis is incomplete, and the fermentation liquid and hydrolyzate coexist in one system, which increases the difficulty of subsequent filtration, separation and purification.

Method used

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  • Method and device for preparing gallic acid
  • Method and device for preparing gallic acid
  • Method and device for preparing gallic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for preparing gallic acid of the present embodiment comprises the following steps:

[0029] (1) Preparation of immobilized tannase

[0030] a. Carrier soaking: Put the carrier LX-1000HA resin in the carrier pretreatment tank with agitator, add deionized water (just submerge the resin on the water surface) for soaking, add NaOH solution with a concentration of 2mol / L to adjust the soaking liquid The pH is 8, and the soaking water is discharged after soaking for 1.5 hours;

[0031] b. glutaraldehyde crosslinking: add K to the carrier pretreatment tank described in step a 2 HPO 4 , glutaraldehyde and deionized water, so that the mass fraction ratio of the material in the pretreatment tank is: carrier LX-1000HA resin: K 2 HPO 4 : glutaraldehyde: deionized water=10:0.4:1.0:88.6. And adding a NaOH solution with a concentration of 2mol / L to adjust the pH to 7.8 and stirring at a stirring speed of 100r / min, after stirring for 1 hour, take out the LX-1000HA resin...

Embodiment 2-6

[0039]The operating steps of Examples 2-6 are the same as those of Example 1. The glutaraldehyde crosslinking process conditions in step (1) and the immobilization conditions of tannase are shown in Table 1. The tannic acid in step (2) See Table 2 for the process conditions of enzymatic hydrolysis and the conversion rate of tannic acid.

[0040]

[0041] Step (2) In the process of preparing gallic acid by tannic acid enzymatic method, the immobilized tannase added is prepared according to Example 2 in step (1), and other process condition parameters are shown in Table 2, and the rest are the same as in Example 1 。

[0042]

Embodiment 8

[0044] With reference to the accompanying drawings, the device for preparing gallic acid in this embodiment includes a tannase immobilization device and a gallic acid preparation device, and the tannase immobilization device includes a carrier pretreatment tank 1, a feed pump I2, Storage tank I3, feeding pump II4, storage tank II5, feeding pump III6, immobilization tank 7, heating and cooling system (not shown in the figure), storage tank I3 is connected with carrier pretreatment tank 1 and The immobilization tank 7 is connected, the storage tank II5 is connected with the carrier pretreatment tank 1 and the immobilization tank 7 through the feed pump II4, the carrier pretreatment tank 1 is connected with the immobilization tank 7 through the feed pump III6, and the carrier pretreatment tank 1, immobilization tank 7 is connected with heating and cooling system; The preparation device of described gallic acid comprises raw material extraction tank 8, feeding pump IV 9, filter pre...

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Abstract

The invention relates to a method and a device for preparing gallic acid. The method for preparing the gallic acid comprises the following steps of: (1) preparation of immobilized tannase: an immobilized vector of tannase is soaked with deionized water, and is cross-linked with glutaraldehyde, and then the tannase is added to complete the immobilization of the tannase; and (2) preparation of the gallic acid: a raw material containing tannin is leached with water and is filtered, and then the immobilized tannase is added into a tannin leaching liquid to be subjected to enzymatic hydrolysis, bleaching, filtering, concentration, crystallization, centrifugation and vacuum drying to obtain the gallic acid. The invention also comprises a device for preparing the gallic acid, which comprises a tannase immobilizing device and a gallic acid preparing device. In the invention, by immobilizing the tannase, the thermal stability and the pH application range of the enzyme are improved; the operation is simple, safe and stable; and the production cost is low, and the large-scale production is convenient.

Description

technical field [0001] The invention relates to a method and a device for preparing gallic acid, in particular to a method and a device for preparing gallic acid by an enzymatic method. Background technique [0002] Gallic acid, the chemical name is 3,4,5-trihydroxybenzoic acid (English name 3,4,5-trihydroxy benzoic acid) is an important fine chemical product, widely used in chemical, pharmaceutical, food and electronic industries, etc. field. [0003] At present, in industry, the raw materials for producing gallic acid are gallnut, beihua or tara, and the main production method is acid hydrolysis or alkali hydrolysis. These methods have the following problems: (1) the concentrated sulfuric acid used in the acid hydrolysis method has strong oxidizing properties, which can destroy the benzene ring structure in tannic acid, and there are many side reactions, which cause the gallic acid yield to decrease, and the crude product color becomes dark; ( 2) Alkaline hydrolysis need...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/40C12P7/42C12N11/08
CPCC12M47/12C12M41/12C12M47/14C12M41/26C12M25/16C12M21/18C12M47/10
Inventor 黎继烈李忠海王挥沈珺珺袁强朱晓媛
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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