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Nematicidal extract of radix-polygoni multiflori and preparation method thereof

A technology of Polygonum multiflorum and nematodes, which is applied to botany equipment and methods, nematicides, biocides, etc., and can solve the problems of prevention and control of plant root-knot nematodes without ethyl acetate extract of Polygonum multiflorum

Inactive Publication Date: 2012-01-18
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report on the application in phytomedicine, and there is no relevant report on the use of ethyl acetate extract of Polygonum multiflorum for the prevention and treatment of plant root-knot nematode

Method used

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  • Nematicidal extract of radix-polygoni multiflori and preparation method thereof
  • Nematicidal extract of radix-polygoni multiflori and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the acquisition of Polygonum multiflorum ethyl acetate extract

[0027] High-speed pulverizer, type 6202, manufactured by Beijing Huanya Tianyuan Machinery Technology Co., Ltd.; desktop three-frequency digital control ultrasonic cleaner, type KQ-500VDV, Kunshan Ultrasonic Instrument Co., Ltd.; rotary evaporator, type RE-2000, Shanghai Yarong Biochemical Co., Ltd. Instrument factory; stereo microscope, Motic SMZ-140, Motic Industrial Group Co., Ltd.; micro-adjustable pipette, GILSON, France; 96-well disposable cell culture plate, JET BIOFIL type, Canada; electric constant temperature incubator, SKP-02.420 type, Huangshi Hengfeng Medical Equipment Co., Ltd.; ethyl acetate (analytical pure), Beijing Chemical Plant;

[0028] Polygontm multiflorum for testing is purchased from Beijing Tongrentang Pharmacy, belongs to Polygonaceae, and uses tubers.

[0029] For the test root-knot nematode (Meloidogyne spp.), the diseased root of tomato root-knot nematode disea...

Embodiment 2

[0044] Embodiment 2, poisonous activity assay method

[0045] The contact method is used for determination, and the specific steps are: take a 96-well cell culture plate, add 150 μL of the plant extract solution obtained by the method 1 of step 2 of Example 1 with a concentration of 20 mg / mL into each well, and then add an equal volume of The root-knot nematode second-instar larvae suspension obtained in step 1 was allowed to stand at 25°C, and observed under a stereomicroscope after 12h, 24h and 48h, respectively, to check whether the nematodes were dead or alive (needle contact method), stiff or curled and motionless worm bodies As death, the corrected death rate of nematodes was calculated according to the formula. With sterile water as the control, each treatment was repeated 3 times, and the results were average ± standard error.

[0046]

[0047]

[0048]Data processing and analysis: The experimental data was analyzed and sorted by DPS2000, and the results were an...

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Abstract

The invention discloses a nematicidal extract of radix-polygoni multiflori and a preparation method thereof, and provides a method for preparing ethyl acetate extract of radix-polygoni multiflori. The method comprises the following steps: 1) uniformly mixing radix-polygoni multiflori with ethyl acetate to obtain a mixture; and 2) ultrasonically treating the mixture obtained in the step 1), carrying out suction filtration and concentrating to obtain the ethyl acetate extract of radix-polygoni multiflori. The experiments prove that radix-polygoni multiflori is extracted by using ethyl acetate, the poisoning and killing effect of the extract on root-knot nematode is tested, and the test result shows that the extract has strong poisoning and killing activity on root-knot nematode; and the extract disclosed by the invention lays a foundation for the subsequent development and application of root-knot nematode biological prevention and control agents. The method has the advantages that nematicidal active substance is extracted by utilizing natural harmless plant materials, pollution caused by chemical pesticides is reduced, and the extraction method is simple and can be easily popularized and applied to the prevention and control of plant root-knot nematode disease.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nematicidal extract of Polygonum multiflorum and a preparation method thereof. Background technique [0002] Root-knot nematodes (Meloidogyne spp.) are obligate parasites on plant roots and are an important pathogen affecting agricultural production. [0003] Since the 1980s, the research on the use of plant extracts to control nematodes has gradually attracted people's attention. Up to now, there are about 102 families, 226 genera, and 316 species of nematode-killing plants, among which Asteraceae, Meliaceae, Bean family, Brassicaceae, Solanaceae, Poaceae, Euphorbiaceae, Poppyaceae, Apocynaceae, Mimosaceae, Liliaceae, etc., but the plant resources in nature are extremely rich, and many plants with nematicidal effects have not been discovered , the book "Poisonous Plants in China" has listed more than 1300 kinds of poisonous plants, many of which have insecticidal activity. Many ...

Claims

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Application Information

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IPC IPC(8): A01N65/30A01P5/00
Inventor 魏艳敏马喆刘正坪尚巧霞赵晓燕宋庆丰
Owner BEIJING UNIV OF AGRI
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