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Method for purification culture of sweet potato rot stem nematodes

A technology for stem nematodes and sweet potatoes, which is applied in the field of high-quality purification and cultivation of D. destructor populations, can solve the problems of lowering the culture purity, complicated methods, and increased workload, and achieves the effects of high population purity, simple methods, and easy operation

Active Publication Date: 2012-01-18
JIANGSU XUHUAI DISTRICT HUAIYIN AGRI SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual application of the above method, if the nematode population is not high in purity and carries other soil nematodes, the workload required to remove these miscellaneous insects will inevitably increase, and the miscellaneous insects will inevitably be carried, reducing the purity of the culture. The purification of nematodes brings inconvenience, and although the above-mentioned purification, culture and propagation methods bring convenience to the rapid and large-scale reproduction of nematodes, if D. Biological changes
Ruan Long et al. reported that the method of propagating D. destructor in potato hairy root single host culture system not only has the above-mentioned defects, but also the method is very complicated.

Method used

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  • Method for purification culture of sweet potato rot stem nematodes
  • Method for purification culture of sweet potato rot stem nematodes
  • Method for purification culture of sweet potato rot stem nematodes

Examples

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Embodiment 1

[0029] The Ditylenchus rotting population in this example was provided by the Nematode Laboratory of the College of Plant Protection, Nanjing Agricultural University. The tuber variety used was Suyu 303 (non-resistant to Ditylenchus rot), provided by the Jiangsu Academy of Agricultural Sciences.

[0030] A method for purifying and cultivating Ditylenchus rotundus includes the following steps:

[0031] (1) Preparation of nematodes

[0032] Use a needle to pick the females of the Ditylenchus decomposed population under a 40× stereomicroscope and place them on a glass slide. Under a 100× microscope, they are further confirmed to be pregnant females, and then placed in a concave dish for later use;

[0033] (2) Purification culture

[0034] Cut off one third of the whole potato lengthwise (to get one third of the potato wedges 1, two thirds of the potato wedges 2), and punch holes in the section of the two thirds of the potato wedges (such as figure 1 As shown, the diameter of the hole 3 ...

Embodiment 2

[0038] A method for purifying and cultivating Ditylenchus rotundus includes the following steps:

[0039] (1) Preparation of nematodes

[0040] Under a 40× stereomicroscope, use a needle to pick the older female and male worms of Ditylenchus rotundus and place them in a container for later use;

[0041] (2) Purification culture

[0042] Cut off one third of the whole potato lengthwise (to get one third of the potato wedges 1, two thirds of the potato wedges 2), and punch holes in the section of the two thirds of the potato wedges (such as figure 1 As shown, the diameter of the hole 3 on the section of the sweet potato is 3 mm, the depth is 20 mm, and the distance between the holes is 3 cm), inject a little sterile water into each hole, as long as the hole does not overflow, then the steps (1) Obtained Ditylenchus rotting, one older larvae female and two males were inserted into a small hole, and then one third of the potato pieces 1 was used as a natural moisturizing cover to cover t...

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Abstract

The invention discloses a method for purification culture of sweet potato rot stem nematodes, which comprises the following steps of: (1) picking older young female and male nematodes in the rot stem nematode by a needle and placing on a container standby; and (2) transversely or vertically cutting the whole potato, perforating holes on one cross section, injecting little sterile water into each hole in a standard that the water does not overflow from the holes, then, inoculating the rot stem nematode, one older young female and two males, obtained in the first step into the small holes, then, covering the cut potato blocks onto the potato blocks inoculated with the rot stem nematode as a natural moisture preservation cover, sealing the edge gaps by paraffin wax, placing the potato blocks into a culture box after being bound by elastic materials, carrying out culture for 30 days at 25 DEG C, and obtaining a plurality of pure rot stem nematodes. The method provided by the invention is simple, the operation is easy, the workload is low, more importantly, the purity of the obtained group is high, the vitality is good, and the assurance is provided for the systematic study work on the biological characteristics, the pathogenic mechanisms, the population groups, the individual genetic variation and the like of the nematode.

Description

Technical field [0001] The present invention is a method of plant disease in the field of biology, which involves high -quality purification cultivation of rotten stem nematodes. technical background [0002] Sweet potato rotten stem neck disease is an important disease in my country's agriculture. It is mainly distributed in Henan, Hebei, Shandong, Shanxi, Anhui, Jiangsu and other provinces in China.Its pathogenic sweet potato rotten stem neck ( DityLenchus Destrus ) It was first discovered on potatoes that caused potato rot (Thorne G, proposed in 1945).Sweet potato stem neck disease is one of the important diseases of the sweet potato production area in my country. The pathogenic rotten stem neck is an important quarantine object in my country.In recent years, due to improper production of sweet potato production, the spread of sweet potato width disease has spread a large area.The harm is getting worse, and the production of sweet potatoes has been endangered.The disease is ca...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K1/18A23K1/14A23K10/30A23K50/00
Inventor 王宏宝梁照文李茹
Owner JIANGSU XUHUAI DISTRICT HUAIYIN AGRI SCI RES INST
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