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Food composition and medicinal composition of lactobacillus strain for treating allergy

A pharmaceutical composition and composition technology, applied in the field of food composition and pharmaceutical composition

Active Publication Date: 2013-04-03
锦乔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of probiotics affecting the human body is still inconclusive. For this point, in the future development, more in vitro and in vivo experiments are still needed to explain why the mechanism of probiotics has such a good effect on the human body and how the research is applied to the human body. Optimal conditions on (Michael et al., 2008)

Method used

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  • Food composition and medicinal composition of lactobacillus strain for treating allergy
  • Food composition and medicinal composition of lactobacillus strain for treating allergy
  • Food composition and medicinal composition of lactobacillus strain for treating allergy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] According to the results of 16S rDNA sequence analysis and API bacterial identification system analysis, the taxonomic characteristics of the strains were confirmed. It was found that Fenghua strain number GL-104 is Lactobacillus reuteri. The characteristics of this strain are listed in Table 2 in detail in terms of morphology and general properties:

[0024] Table 2

[0025] strain name Morphological characteristics Lactobacillus reuteri GL-104 1. When cultured in MRS medium, the bacteria are in the shape of short rods with rounded ends, usually appearing alone, in pairs or in short chains. 2. Gram-positive bacilli do not produce spores, do not have catalase, oxidase and motility, and can grow in both aerobic and anaerobic environments. The most suitable growth temperature is 37±1°C, which belongs to facultative heterogeneity A fermentative strain that does not produce gas during glucose metabolism.

[0026] Example 2: The effect of anti-alle...

Embodiment 2

[0027] To examine the effect of the anti-allergic lactic acid bacteria strain, Lactobacillus reuteri GL-104 strain M 209138, on the Th1 type immunity enhancement effect, which is to measure the mouse spleen cells (Splenocyte) and human peripheral blood mononuclear cells (Peripheral blood mononuclear cell , PBMC) and the aforementioned strain of anti-allergic lactic acid bacteria were respectively co-cultured to measure the secretion of interferon gamma to verify the strain with anti-allergic ability. Use the following experimental procedure:

[0028] 1. Take an appropriate amount of mouse spleen and human blood, about one spleen and 200mL whole blood each time.

[0029] 2. Put the spleen into the buffer solution and crush it, centrifuge at 100g for 5 minutes, and take the supernatant for later use.

[0030] 3. Take an equal proportion of blood cell separation solution (Ficoll-paque), spleen cell supernatant and blood and centrifuge at 400g for 30-40 minutes at 18-20°C.

[00...

Embodiment 3

[0041] To examine the enhancement effect of the anti-allergic lactic acid bacteria strain of the present invention-Lactobacillus reuteri GL-104 strain M209138 on Th1-type immunity, it is determined that human dendritic cells (dendritic cells) are co-cultivated with the aforementioned anti-allergic lactic acid bacteria The secretion of interleukin 12p40 (IL-12p40) was measured to verify the strain with anti-allergic ability. Use the following experimental procedure:

[0042] 1. Draw an appropriate amount of human blood about 200mL each time.

[0043] 2. Take an equal proportion of Ficoll-paque and blood and centrifuge at 400g for 30-40 minutes at 18-20°C.

[0044] 3. Take the human peripheral blood mononuclear cell (PBMC) layer, wash the cells with buffer solution for 2-3 times, and suspend the cells with an appropriate medium (such as RPMI-1640).

[0045] 4. Human peripheral blood mononuclear cells (PBMC) cells were infected with CD14 + Microbeads (MiniMACSsystem) will CD14...

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Abstract

The invention relates to a novel lactobacillus strain and a novel composition which contains an unknown strain which can be used for enhancing the anti-allergic capability. The composition can exist in the form of food or medicinal composition. A method for screening and testing the lactobacillus strain composition for enhancing the Th1 immunological competence and regulate overreacting Th2 immunologic reaction caused by allergy is also disclosed.

Description

technical field [0001] The invention relates to a food composition and a pharmaceutical composition, in particular to a food composition and a pharmaceutical composition containing anti-allergic lactic acid bacteria strains. Background technique [0002] Generally, consuming products containing lactic acid bacteria (LAB) only has the effect of adjusting the health of the intestinal tract. Although tens of thousands of lactic acid bacteria strains exist in nature, only a few lactic acid bacteria strains have anti-allergic properties. A small number of these bacterial strains have the characteristics of acid resistance and bile salt resistance, the ability to absorb mucosal epidermal cells, and the ability to survive after passing through the gastrointestinal tract, which is an important basis for screening strains with health-promoting effects. Up to now, only a few strains of lactic acid bacteria that have been proven to have anti-allergic health effects have been identified...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L1/30A61K35/74A61P37/08A61K35/747
Inventor 谢佩珊蔡宜钧郭仲伟
Owner 锦乔生物科技有限公司
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