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Protein GmMYB118 related to fatty acid synthesis, coding gene and application thereof

A technology for encoding genes and fatty acids, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as the inability to effectively improve the content of fatty acids, achieve broad application and market prospects, and improve the effect of content

Active Publication Date: 2013-06-12
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past ten years, scientists have used biotechnology to genetically improve the key genes in the process of fatty acid biosynthesis, but have failed to achieve significant results. One of the main reasons is that fatty acid synthesis in plants is a complex and highly coordinated process. The process is completed by multiple genes, so improving a single gene for fatty acid synthesis cannot effectively improve the content of fatty acids

Method used

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  • Protein GmMYB118 related to fatty acid synthesis, coding gene and application thereof
  • Protein GmMYB118 related to fatty acid synthesis, coding gene and application thereof
  • Protein GmMYB118 related to fatty acid synthesis, coding gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1, discovery of gene

[0041] The homologous gene GmMYB118 of Arabidopsis thaliana AtMYB118 gene was found in soybean by homologous sequence comparison. In the sequence alignment, it was found that the conserved domain of soybean GmMYB118 gene and Arabidopsis AtMYB118 gene reached 42% homology.

Embodiment 2

[0042] Embodiment 2, the acquisition of transgenic plants and its detection

[0043] 1. Obtaining of transgenic plants

[0044] 1. Construction of recombinant expression vector

[0045] Soybean (Glycine max cultivar Nannong11382) (the public can obtain from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, the non-patent literature that has recorded this material is LiaoY, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang JS* and Chen SY*. Soybean GmbZIP44, GmbZIP62and GmbZIP 78genes function as negative regulator of ABA signaling and conf er salt and freezing tolerance in transgenic Arabidopsis. Planta. 2008, 228: 225-240) leaf genomic DNA as template , with the following primers:

[0046] GmMYB 118-F: CCctcgagATGTTCAAAGAAAGCTATGTCTCA (lowercase letter is the restriction site of XhoI) (sequence 5 in the sequence listing) and

[0047] GmMYB118-R: GGcccgggCACACGAAGAATTTCATTACTAAT (lowercase letters are the SmaI restriction site) (sequence...

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Abstract

The invention discloses protein GmMYB118 related to fatty acid synthesis, coding gene and an application thereof. The protein related to the fatty acid synthesis is named as GmMYB118, and is derived from soybean (Glycine max L.). The protein is the following protein represented by 1) or 2): 1) the protein comprises amino acid sequence represented by a SEQ ID No.1 in the sequence table; 2) the protein is derived from the SEQ ID No.1 [protein (1)] and is related to the vegetable fatty acid synthesis, wherein the amino acid sequence residues represented by the SEQ ID NO.1 are processed from substituting, and / or deleting, and / or adding one or a plurality of amino acid residues. According to the present invention, after the gene GmMYB118 provided by the present invention is overexpressed in arabidopsis thaliana, the fatty acid content in the arabidopsis thaliana seedling can be improved, such that the important theoretical and practical meaning is provided for improvement of the fatty acidcontent in the soybean and the improvement of related characteristics, and the wide application and market prospect is provided for the agricultural field.

Description

technical field [0001] The invention relates to protein GmMYB118 related to fatty acid synthesis in the field of biotechnology, its coding gene and application. Background technique [0002] Vegetable oil has attracted extensive attention as a major edible oil and an important renewable energy substance. At present, 85% of the world's edible oil comes from vegetable oil, and only 25% comes from animals and marine organisms. At the same time, vegetable oil is also widely used in industrial fields, such as detergents, lubricants, paints and biodegradable plastics. In addition, it is worth mentioning that the development of vegetable diesel may provide a new way to solve the global energy crisis and environmental pollution. As people's demand for vegetable oil is increasing, the existing production will be far from meeting the needs of society. [0003] As one of the main economic crops, soybean is an important source of edible vegetable oil. Compared with other vegetable o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21C12N15/82C12N15/84A01H5/00
Inventor 梁岩牟金叶左建儒
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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