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Preparation method of gnaphalium affine total flavonoid

A technology of total flavonoids and sagebrush, applied in botany equipment and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of environmental impact, large amount of organic solvents, and various types, etc., and achieve resin treatment The effect of large amount, strong extraction ability and high extraction rate

Inactive Publication Date: 2012-01-04
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the amount of organic solvents is large and there are many types, which has a great impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Crush the medicinal material of sagebrush, take 1kg, put it into the extraction kettle, use supercritical carbon dioxide to extract, control the extraction pressure to 28MPa, the extraction temperature to 60°C, and the flow rate of carbon dioxide to be 18L / h, the entrainer It is 60% ethanol, the dosage is 2L, extract for 2 hours, after the extraction is finished, the extract enters the analysis kettle, set the pressure of the analysis kettle to 10MPa, and the temperature to 24°C, then collect the analysis solution from the analysis kettle, add to the analysis solution The same amount of petroleum ether was extracted 5 times, the raffinate was collected, and the petroleum ether was evaporated;

[0016] Take 350g of 100-mesh polyamide resin, pack it into a column, put the raffinate on the column, elute with 3BV of 65% ethanol, concentrate the eluent, and dry to obtain 33.7g of the total flavonoids product of S. japonicus, with a content of 86.6%.

Embodiment 2

[0018] Crush the medicinal material of sagebrush, take 1kg, put it into the extraction kettle, use supercritical carbon dioxide to extract, control the extraction pressure to 45MPa, the extraction temperature to 30°C, and the flow rate of carbon dioxide to be 40L / h, the entrainer It is 80% ethanol, the dosage is 6L, and the extraction is 3h. After the extraction is completed, the extract enters the analysis kettle. Set the pressure of the analysis kettle to 12MPa and the temperature to 20°C. Then collect the analysis solution from the analysis kettle and add it to the analysis solution. The same amount of petroleum ether was extracted 6 times, the raffinate was collected, and the petroleum ether was evaporated;

[0019] Take 500g of 200-mesh polyamide resin, pack it into a column, put the raffinate on the column, elute with 5BV of 75% ethanol, concentrate the eluent, and dry to obtain 27.7g of the total flavonoids product of S. japonicus, with a content of 89.1%.

Embodiment 3

[0021] Crush the medicinal material of sage clam, take 2kg, put it into the extraction kettle, use supercritical carbon dioxide to extract, control the extraction pressure to 30MPa, the extraction temperature to 40°C, and the flow rate of carbon dioxide to be 25L / h, the entrainer It is ethanol, the dosage is 7.5L, and the extraction is 3h. After the extraction is completed, the extract enters the analysis kettle. Set the pressure of the analysis kettle to 7MPa and the temperature to 28°C. Then collect the analysis solution from the analysis kettle and add it to the analysis solution. The amount of petroleum ether was extracted 3 times, the raffinate was collected, and the petroleum ether was evaporated;

[0022] Take 1000g of 100-mesh polyamide resin, pack it into a column, put the raffinate on the column, elute with 80% ethanol of 8BV, concentrate the eluent, and dry to obtain 53.4g of the total flavonoids product of S. japonicus, with a content of 81.5%.

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Abstract

The invention provides a preparation method of gnaphalium affine total flavonoid, and particularly relates to an method for extracting total flavonoid from plants. The method comprises the following steps: crushing gnaphalium affine medicinal material; extracting and analyzing by adopting supercritical carbon dioxide; adding petroleum ether into analytical liquid for multiple extraction and impurity removal; distilling the residual extraction liquid to remove petroleum ether, and adsorbing through polyamide resin; eluting with ethanol solution, and concentrating the eluate; and drying to obtain the gnaphalium affine total flavonoid product. The preparation method has the advantages of simple process, complete extraction, high efficiency and energy conservation, and is suitable for industrial production in large scale.

Description

technical field [0001] The invention relates to the field of separation of natural products, in particular to a preparation method of total flavonoids of sagebrush. Background technique [0002] Sage ( Gnaphlium affine D.Don), also known as Vermicelli, Qingmingcai, is a plant of the genus Salmonella in the Chrysanthemum family. The grassland name "Mouse Ear" was first recorded in "Bielu of Famous Doctors". "Supplements to Materia Medica" claims that "Chrysanthemum chinensis, born in the hills and cultivated land, more than a foot high, with white hairs on the leaves and yellow flowers", has been included in the 1977 edition of "The Pharmacopoeia of the People's Republic of China". According to the determination, the whole herb of Shamrock contains 5% flavonoid glycosides. Flavonoids have anti-oxidation, anti-allergic, anti-inflammatory, anti-bacterial, anti-mutation, anti-tumor, hepatoprotective effects, protection of cardiovascular and cerebrovascular systems, anti-viru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A01N65/12A01P7/04A61P11/10A61P29/00A61P31/04A61P39/06
Inventor 刘东锋吴艳波
Owner NANJING ZELANG MEDICAL TECH
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