Composite microcapsule model for use in in-vitro cell coculture

Abstract

The invention discloses a composite microcapsule model for use in in-vitro cell coculture. The model is a sphere-in-sphere composite microcapsule model consisting of an inner sodium alginate gel microsphere and an outer sodium alginate gel microsphere, which is formed by using natural polysaccharide sodium alginate as a substrate material, dripping the polysaccharide sodium alginate into multivalent cation solution and performing gelatinization treatment twice, wherein the two gel microspheres may contain two different kinds of cells. The model can be used for the study on in-vitro coculture of various cells and overcomes the drawback that the interaction between cells of the same kinds and the interaction between cells of different kinds cannot be controlled, the drawback that different kinds of cells are difficult to separate in late-stage study and the drawback that cell dependency exists of the conventional coculture model; and the model can simulate cell-cell interaction, cell-extracellular matrix interaction and cell-soluble factor interaction and provide theoretical and technical support for in-vitro construction of a tumor model, optimization of in-vitro culture mode of cells and tissue engineering construction of various tissue and organ analogues or equivalents.

Description

technical field [0001] The invention belongs to the field of cell in vitro culture technology, in particular to an animal cell in vitro co-culture model. Background technique [0002] In the body, cells are in a highly informational microenvironment filled with various complex physical and chemical signals, and cells respond to various signal stimuli to achieve their specific biological functions. The interaction between cells and this microenvironment includes various complex biochemical, biomechanical and bioelectrical signals from surrounding cells, extracellular matrix and soluble factors. Among them, the interaction between cells occurs through direct contact or paracrine soluble factors. This kind of intercellular communication is an important part of maintaining the structure and function of cells, tissues and organs, and it is also a key factor in regulating tissue repair and reconstruction in vivo and in vitro, and the lack of this kind of communication is the los...

AI Technical Summary

Problems solved by technology

However, this co-cultivation method is relatively complicated to operate, and the preparation process of the substrate material is relatively cumbersome, and it is limited to the co-cultivation of adherent cells, which greatly limits its application range.

At the same time, cells are still in a two-dimensional culture environment in this system, and the two-dimensional cell culture method cannot provide a three-dimensional space microenvironment similar to that required for cell growth in vivo, which will adversely affect many biological characteristics such as cell morphology and functional expression.

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