A Strain of Enterococcus Faecalis for Feed and Its Application

A technology of Enterococcus faecalis and Escherichia coli, which is applied in the fields of application, bacteria, animal feed, etc., to achieve the effects of enhancing animal disease resistance, reducing feed-to-meat ratio, and broad application prospects

Inactive Publication Date: 2011-12-14
北京金泰得生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Enterococcus faecalis disclosed in the above-mentioned patent documents are all researches on the properties of Enterococcus faecalis against bile salts and gastric acid and its application method in piglet feed, and there is no research on its antibacterial effect

Method used

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  • A Strain of Enterococcus Faecalis for Feed and Its Application
  • A Strain of Enterococcus Faecalis for Feed and Its Application
  • A Strain of Enterococcus Faecalis for Feed and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation, Identification and Preservation of Enterococcus faecalis SBD CGMCC No.4848

[0033] 1. Isolation of Enterococcus faecalis SBD CGMCC No.4848

[0034] MRS liquid medium: glucose 20g / L, peptone 10g / L, yeast extract 5g / L, diammonium citrate 2g / L, sodium acetate 5g / L, beef extract 10g / L, Tween 801g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O0.058g / L, MnSO 4 4H 2 O 0.25g / L, pH 6.8.

[0035] Enrichment medium: liquid MRS medium containing 40 μg / mL cycloheximide.

[0036] CaCO 3 MRS medium: MRS solid medium containing 0.5% (mass percentage) light calcium carbonate.

[0037] Acid-resistant MRS medium: Adjust the pH of the MRS liquid medium to 3.0.

[0038] Bile salt-tolerant screening medium: add 0.3% (mass percentage) pig bile salt to the MRS medium.

[0039] The fresh feces of sows were collected from the Breeding Pig Testing Center of Huazhong Agricultural University, enriched with enriched culture medium (the culture condition was 37°C, static culture),...

Embodiment 2

[0048] Embodiment 2, analysis of the gastric juice resistance property of Enterococcus faecalis (Enterococcus faecalis) SBD CGMCC No.4848

[0049] Preparation of synthetic gastric juice: tryptone 8.3g, glucose 3.5g, sodium chloride 2.05g, calcium chloride 0.11g, potassium chloride 0.37g, potassium dihydrogen phosphate 0.6g, pig bile salt 0.05g, lysozyme 0.1g, stomach Add 13.3 mg of protease and 1000 mL of distilled water, adjust the pH values ​​to 1.5 and 2.5 with 1 mol / L hydrochloric acid, and sterilize at 115°C for 20 minutes. Among them, lysozyme and pepsin were sterilized by filtration.

[0050] To activate the strain, transfer Enterococcus faecalis (Enterococcus faecalis) SBD CGMCCNo.4848 into 100mL liquid MS medium according to the inoculum size of 1%, cultivate overnight at 37°C and 200r / min, take 1mL of bacterial liquid and add 9mL of aseptic synthetic In the gastric juice, the number of viable bacteria was detected by plate counting method at 0 min, 30 min, 60 min, a...

Embodiment 3

[0052] Example 3, analysis of bile salt resistance properties of Enterococcus faecalis (Enterococcus faecalis) SBD CGMCC No.4848

[0053] Prepare MRS liquid medium, which is added with 0.1%, 0.3%, 0.5%, 1.0%, 2.0%, 3.0%, 5.0% (mass percentage concentration) bile salt (purchased from Sinopharm Pharmaceutical Co., Ltd.). Take 1mL 37 ℃ overnight cultivation of Enterococcus faecalis (Enterococcus faecalis) SBD CGMCC No.4848 bacterial cell fermentation broth (10 9 CFU / mL), added to 9mL MRS liquid medium supplemented with the above concentrations of bile salts, cultured at 37°C for 12h, diluted 10 4 After doubling, take 0.1mL solid agar plate coated with MRS, and observe the growth of the bacteria to detect its bile salt tolerance. If the number of growth exceeds 30 (the number of bacteria is 3×10 6 CFU / mL) indicates that it grows well and can tolerate the concentration of bile salts; if the growth number is 5-30, it indicates that the growth is poor, and the ability to tolerate t...

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Abstract

The invention discloses an Enterococcus faecalis strain SBD, of which the collection number is CGMCC No.4848. The strain is a Gram positive strain and is spherical according to observation with microscope, and the strain does not form spores; the strain can well grow on a Mann, Rogosa and Sharpe (MRS) agar plate and can grow into a round, smooth and raised bacterial colony like a grey white dew and with a diameter of 0.5 to 1 millimeter within 48 hours; and the strain can grow in a facultatively anaerobic environment, the growth temperature range is from 10 to 50 DEG C, the optimal growth temperature is 30 to 40 DEG C, and the growth pH value range is from 4 to 10 and the optimal pH value is 6.5. The bacterial preparation made by the strain is nontoxic and safe, can resist gastric juice and cholate, has a strong inhibition effect on various harmful bacteria and can be widely used in livestock breeding industry (by directly adding into daily ration or drinking water of animals) to increase the disease resistance in animals; and the preparation is expected to replace antibiotic for feed purpose and can obviously improve average weight of weaned piglets, reduce a feed-to-meat ratio and improve living environment of livestock and therefore has a bright prospect.

Description

technical field [0001] The invention belongs to microbial strains, in particular to an Enterococcus faecalis strain and its application in poultry and livestock breeding instead of antibiotics. Background technique [0002] Lactic acid bacteria are the resident flora of the intestinal tract. After animals take lactic acid bacteria, they can change the intestinal flora environment, produce a variety of antibacterial factors, inhibit the reproduction of harmful bacteria, and improve the immunity of animals. Lactic acid bacteria are closely combined with intestinal mucosal cells through adhesin, colonize and occupy space on the intestinal mucosal surface, and become the main component of the physiological barrier, so as to restore host resistance, repair the intestinal flora barrier, and cure intestinal diseases. [0003] There are countless experimental reports on the effects of lactic acid bacteria at home and abroad, and the view that lactic acid bacteria are beneficial bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A61K35/74A61P31/04C12R1/01A23K10/18A61K35/742
Inventor 雷红升梁运祥赵述淼张东晓唐泽华沈中艳付生慧游伟方莉玉
Owner 北京金泰得生物科技股份有限公司
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