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Cultivating method of Hosta plantaginea Gold Standard

A cultivation method, the technology of the golden flag, is applied in the field of tissue culture of herbaceous plants to achieve the effect of increasing the survival rate, increasing the yield, and increasing the speed of reproduction

Inactive Publication Date: 2013-07-24
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The report of tissue culture of Hosta 'Golden Flag' has not been seen in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of cultivation method of hosta golden flag, the method comprises the following steps:

[0019] (1) Aseptic treatment

[0020] The small buds of hosta "Jinqi" dug in early spring were rinsed with tap water for 2 hours and then placed on an ultra-clean workbench, soaked in ethanol with a concentration of 75wt% for 30s, soaked in mercuric chloride solution of 1wt‰ for 15min, and rinsed with sterile water for 5- After 6 times, dry the surface water with sterile filter paper, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA5.0mg / L+NAA0.5mg / L;

[0021] (2) Differentiation and proliferation of buds

[0022] 3 weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. After culturing for 1 month, the callus with buds was cut and placed Proliferation medium MS+6-BA2.0mg / L+NAA0.2...

Embodiment 2

[0030] A kind of cultivation method of hosta golden flag, the method comprises the following steps:

[0031] (1) Aseptic treatment

[0032] The small buds of hosta "Jinqi" dug in early spring were rinsed with tap water for 2 hours and then placed on the ultra-clean workbench, soaked in 75wt% ethanol for 20s, soaked in 1wt‰ mercury chloride solution for 10min, rinsed with sterile water for 5- After 6 times, dry the surface water with sterile filter paper, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;

[0033] (2) Differentiation and proliferation of buds

[0034] 3 weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. After culturing for 1 month, the callus with buds was cut and placed Proliferation medium MS+6-BA1.0mg / L+NAA0.1mg / L was cultured, and there we...

Embodiment 3

[0042] A kind of cultivation method of hosta golden flag, the method comprises the following steps:

[0043] (1) Aseptic treatment

[0044] The small buds of hosta "Jinqi" dug in early spring were rinsed with tap water for 2 hours and then placed on the ultra-clean workbench, soaked in 75wt% ethanol for 40s, 1wt‰ of mercury chloride solution for 20mins, rinsed with sterile water for 5- After 6 times, dry the surface water with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;

[0045] (2) Differentiation and proliferation of buds

[0046] 3 weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. After culturing for 1 month, the callus with buds was cut and placed Proliferation medium MS+6-BA3.0mg / L+NAA0.3mg / L was cultured, and there were more...

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Abstract

The invention relates to a cultivating method of Hosta plantaginea Gold Standard. The method comprises the following steps of: first carrying out sterile treatment on Hosta plantaginea Gold Standard buds excavated in the early spring; and then inoculating the buds on a bud inducing culture medium to differentiate the buds; culturing the differentiated buds for 1 month; and then cutting off a budding callus and putting the budding callus into a proliferation medium to be proliferated so as to obtain cluster buds; after dividing the cluster buds into shoots or single buds, putting the shoots orthe single buds into a seedling culture medium to culture the seedling with adventitious buds; obtaining 2-3 cm small plants after 20 days; transferring the small plants into a rooting culture mediumto be induced for rooting; selecting strongly growing sterile seedlings with developed roots; carrying out seedling adaptation indoors in an open bottle for three days; washing off agars at the rootsof the seedlings after taking out the seedlings; transplanting the seedlings outdoors after naturalizing the seedlings in a greenhouse for 40 days; and carrying out fertilizer and water management onthe seedlings. Compared with the prior art, the cultivating method of the Hosta plantaginea Gold Standard provided by the invention has the following advantages that: the reproductive speed and the evenness of the nursery-grown plants can be largely increased; the original maternal character is preferably kept; and the survival rate can be increased so that the yield is increased and the transplanting survival rate can reach 95%.

Description

technical field [0001] The invention relates to a method for tissue culture of herbaceous plants, in particular to a method for cultivating the hosta golden flag. Background technique [0002] Hosta perennial herb. Plant height 30cm-50cm. Leaf bases form clumps, ovate to heart-shaped ovate; racemes are terminal, higher than leaf clumps, flowers are white, tubular funnel-shaped, fragrant. The flowering period is from June to August. Hosta 'Jinqi' is a horticultural variety obtained through artificial breeding of Hosta. It has yellow-green leaves, dark green edges, elegant leaf patterns, and good ornamental value. It is strong, cold-tolerant, shade-loving, avoids direct sunlight, and does not choose soil , but it grows luxuriantly in well-drained, fertile and humid places. It is a better shade plant. It can be used as a ground cover under a tree in the garden, or planted in a rock garden or on the north side of a building. It can also be used as a potted ornamental plant or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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