Construction method and use of grass carp matrix metalloproteinase inhibitor-2 gene, encoded recombinant protein and its spatiotemporal expression map

Abstract

The invention discloses the construction method and application of the grass carp matrix metalloproteinase inhibitory factor-2 gene, the encoded recombinant protein and its spatiotemporal expression map. The nucleotide sequence of the grass carp matrix metalloproteinase inhibitor-2 gene of the present invention is shown in SEQ ID NO:1. The amino acid sequence of the recombinant protein encoded by the nucleotide sequence is shown in SEQ ID NO:2. The matrix metalloproteinase inhibitor-2 gene of grass carp of the present invention is expressed in grass carp tissue and development process, and this gene is transcribed in a large amount in blood, mesonephros, head kidney, spleen, gill, intestine, heart, in brain, muscle, liver , There is a small amount of transcription in the skin, and the existence of a small amount of mRNA is detected in the fin rays. The gene was transcribed in a large amount from the caudal fin stage, and reached the maximum expression level on the 3rd day of fry. The invention also discloses the application of the grass carp matrix metalloproteinase inhibitor-2 gene and the coded recombinant protein in raising grass carp seedlings and as a feed additive for grass carp.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and in particular relates to a grass carp matrix metalloproteinase inhibitor of metalloproteinases-2 (grass carp Tissue inhibitor of metalloproteinases-2, gcTIMP-2) gene, a recombinant protein encoded and a construction method for its temporal and spatial expression. Application of grass carp matrix metalloproteinase inhibitor-2 (grass carp Tissue inhibitor of metalloproteinases-2, gcTIMP-2) gene. Background technique [0002] Grass carp is a kind of herbivorous fish with low feeding cost and wide breeding area. It is the main species of freshwater aquaculture in my country, and its output accounts for about 20% of the total freshwater aquaculture output. However, there are many diseases of grass carp, among which hemorrhagic disease of grass carp is the worst, which brings huge losses to the aquaculture industry. At present, the cause of fulminant hemorrhagic disease in aquatic animals ha...

AI Technical Summary

Problems solved by technology

However, grass carp suffers from many diseases, among which the hemorrhagic disease of grass carp is the worst, which brings huge losses to the aquaculture industry.

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