Composition, kit and method for detecting rotavirus group A

A technology of detection wheel and composition, applied in biochemical equipment and methods, recombinant DNA technology, microbial determination/inspection, etc., can solve problems such as insufficient primer specificity, no relevant reports, false negative results, etc., and achieve rapid detection. Simple, Biohazard Reduction, Accurate Results

Active Publication Date: 2011-11-30
成都新基因格生物科技有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

If the primers are not specific enough, it may lead to false positive results or false negative results of the detection
At present

Method used

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  • Composition, kit and method for detecting rotavirus group A
  • Composition, kit and method for detecting rotavirus group A
  • Composition, kit and method for detecting rotavirus group A

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Embodiment Construction

[0030] The present invention will be described in detail below in conjunction with specific embodiments. It should be pointed out that the embodiments listed here are only for illustrative purposes and should not be interpreted as any limitation on the scope of the present invention. The reagent kits, buffers and other reagents used therein are only the reagents specifically selected in the specific embodiment. It should be understood that those skilled in the art can select corresponding reagents from other companies as needed to achieve the purpose of the present invention.

[0031] In order to specifically detect rotavirus group A, the inventors selected its genome-specific sequence as the target sequence, designed specific primers for this sequence, and selected the most specific pair of primers (P1 and P2 through a large number of experiments). , See Table 1) used in the present invention.

[0032] In one embodiment of the present invention, P1 and P2 are selected as specific...

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Abstract

The invention discloses a composition for detecting group A rotavirus, belonging to the technical field of biology, comprising the following primers: 5'-GGCTTTAAAAGAGAGAATTTCC-3' and 5'-AAYTGYGGTATATTCAATACCATACA-3', also comprising the following probes: 5'-fluorescent reporter dye AARGAGCTAWCCGYTAGCCAGACGG-fluorescence quencher-3'. The invention further discloses a kit containing the primers andprobes and a method for detecting group A rotavirus. When using the composition, the kit and the/or method to detect group A rotavirus, a genome equivalent/ reaction system with the range of 10<8>-10<1> copies is detected, the detection is rapid and simple, and the whole detection process can be completed within 3 hours with high specificity and high accuracy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composition, a kit and a detection method for specifically detecting viruses. Background technique [0002] Rotavirus mainly infects human small intestinal epithelial cells, causing cell damage and diarrhea. The course of the disease is generally about one week, fever lasts for 3 days, vomiting for 2-3 days, diarrhea for 5 days, and dehydration symptoms in severe cases. The rotaviruses that cause autumn and winter diarrhea in children mainly belong to group A, while the rotaviruses in adults mostly belong to group B, and groups A and B cannot be distinguished morphologically. Around 900,000 infants and young children die each year from rotavirus group A infection worldwide, most of which occur in developing countries. In my country, there are about 40 million infants under 2 years old, and about 10 million infants suffer from rotavirus infectious gastroenteritis every year, accoun...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11G01N21/64
Inventor 王玲汪耀
Owner 成都新基因格生物科技有限公司
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