A kind of culture medium for fermentative production of bacterial cellulose

A bacterial cellulose and culture medium technology, applied in the field of bacterial cellulose products, can solve the problem of not using coconut meat

Active Publication Date: 2011-11-30
HAINAN YEGUO FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is currently no report on the use of coconut meat as a component of bacterial cellulose fermentation medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] After taking the coconut and removing the coconut coat, breaking the shell to take the meat, then adding 3 weight multiples of hot water at 60°C to the coconut meat, soaking for 60 minutes, and beating with a colloid mill to make a slurry. A liquid medium was prepared according to 80% by weight of the slurry and 20% by weight of sucrose, and finally the pH value was adjusted to 4.0 with glacial acetic acid.

Embodiment 2

[0015] After taking coconut water and removing the coconut coat, breaking the shell to take the meat, then adding 2 weight multiples of hot water at 70°C to the coconut meat, soaking for 45 minutes, and beating with a colloid mill to make a slurry. According to 60% by weight of slurry, 20% by weight of glucose, and 20% by weight of coconut water, a liquid medium is prepared, and finally the pH value is adjusted to 3.5 with citric acid.

Embodiment 3

[0017] After taking the coconut and removing the coconut coat, breaking the shell to take the meat, then adding 1.5 times by weight to the coconut meat, soaking in hot water at 80°C for 30 minutes, and then beating with a colloid mill to make a slurry. According to 40% by weight of slurry, 15% by weight of lactose, 40% by weight of coconut water, 4% by weight of peptone, 0.2% by weight of potassium dihydrogen phosphate, 0.2% by weight of disodium hydrogen phosphate and 0.6% by weight of zinc sulfate, a liquid medium is prepared, and finally Adjust the pH to 5.0 with glacial acetic acid.

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PUM

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Abstract

The invention relates to a culture medium for producing bacterial cellulose through fermentation. The culture medium contains coconut flesh slurry which accounts for 20 to 80 percent of the total weight of the culture medium, wherein the coconut flesh slurry is obtained by adding water into coconut flesh and beating. In the invention, the coconut flesh with higher yield is treated and then is taken as a culture substrate for bacterial cellulose fermentation producing bacteria, and replaces fermentable sugar or coconut water which is cultured and used by the conventional method and is higher in cost, so that the cost is reduced; meanwhile, the application range of the coconut flesh is extended, the production added value of the coconut flesh is improved, and the resource is saved. Coconut flesh tissues contain rich nutrient substances which are adequate to provide a carbon source and a nitrogen source which are needed by the fermentation of the bacterial cellulose, and are good fermentation substrates.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a culture medium for fermenting and producing bacterial cellulose and bacterial cellulose products produced by fermenting the culture medium. Background technique [0002] Bacterial Cellulose (BC) is produced by fermentation of Gluconacetobacter xylinum, etc. Through static fermentation, film-shaped bacterial cellulose products can be produced, and through dynamic fermentation, spherical bacterial cellulose products can be produced. Film-like bacterial cellulose has good water-holding properties, high crystallinity and polymerization degree, and good biocompatibility and biodegradability. It can be widely used in food, medicine, aviation, papermaking and other fields. At present, coconut water is often used as the main component of the culture medium for fermentation to produce bacterial cellulose. Although it is cheap and easy to use, it is easy to spoil and deteriorate, ...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08L1/02C12R1/01
Inventor 钟春燕
Owner HAINAN YEGUO FOODS
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