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A method for producing succinic acid by fermenting distiller's grain raw materials

A technology of succinic acid and distiller's grains, applied in the field of bioengineering, to achieve the effects of shortening the fermentation cycle, improving fermentation efficiency, and increasing reaction speed

Inactive Publication Date: 2011-11-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main components of distiller's grains are about 10% starch, about 20% cellulose, and 7-10% protein, which have the potential to be used as fermentation raw materials for fermentation, but there are no reports on the use of distiller's grains as raw materials to ferment succinic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-2

[0030] Example 1-2 Fermentation of rice distiller's grains to produce succinic acid

[0031] Put the fresh yellow rice distiller's grains in an oven at 105°C, bake for about 8 hours, take them out and crush them to obtain 60-mesh distiller's grains particles. Its starch content is 13.5%, crude fiber is 6.2%, protein is 13.8%, its water content is less than 20%, and the alcohol content is less than 0.005 g alcohol / g distiller's grains.

[0032] Take 50 mL of culture medium, add 20 g and 25 g of rice wine grains respectively, and the phosphate buffer system is K 2 HPO 4 ·3H 2 O 2 g / L, NaH 2 PO 4 2H 2 O 2 g / L, MgCl 2 ·6H 2 O and CaCl 2 1 g / L, sodium acetate 1-3 g / L, pH 7.0, sterilized at 120°C.

[0033] Add commercially available α-amylase (Shandong Jienuo) 50 U / g distiller’s grains, hydrolyze at 90°C for 1.0 h, cool down to 50-60°C, then adjust the pH to 4.8, add commercially available glucoamylase (Genencor) 40 U / g distiller's grains, commercially available cellula...

Embodiment 3-4

[0037] Example 3-4 Fermentation of distiller's grains to produce succinic acid

[0038] Put the fresh distiller's grains into an oven at 105°C, dry them for about 8 hours, take them out and crush them to obtain 40-60 mesh distiller's grains. Its starch content is 10.2%, crude fiber 26.8%, protein 8.2%, its moisture content is less than 20%, and the alcohol content is less than 0.005 g alcohol / g distiller's grains.

[0039] According to the method of Example 1, in each 50 mL shake flask, add 20 g or 25 g distiller's grains respectively to prepare a fermentation medium, add enzymes for two-stage pre-hydrolysis, and insert Actinobacillus succinicum CGMCC at 5% to 10% No.1593, filled with CO 2 , and cultured at 37°C for 72 h. Adjust and maintain the pH of the fermentation broth at 6.0~7.5. Table 2 shows the organic acid content in the shake flask fermentation broth.

[0040] Table 2 Shake bottle fermentation results of distiller's grains

[0041] Distiller's grai...

Embodiment 5

[0042] Extraction of Succinic Acid in Distiller's Grains Fermented Liquid of Embodiment 5

[0043] The fermented broth after distiller's grains fermentation is centrifuged or plate-and-frame press-filtered to obtain the fermented supernatant, and its succinic acid concentration is 30.4 g / L. Take 200 mL of fermentation clear liquid, adjust the pH to 2, add 1.5% (w / v) activated carbon for decolorization, and desalt with 732 cation exchange resin to obtain a desalted liquid with a volume of about 290 mL, and the concentration of succinic acid is 20.5 g / L. Extract with 290 mL extractant (trioctylamine:n-octanol=7:3, v / v) at 40°C for 50 min, and the extract phase contains 17 g / L succinic acid; Then at 40°C, add 1.5 mol / L sodium carbonate solution according to the ratio of extraction phase: sodium carbonate solution volume ratio 2:1, carry out back extraction for 60 min, separate liquid after back extraction, and measure the The concentration of succinic acid was 31 g / L; then the...

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PUM

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Abstract

The invention discloses a method for producing succinic acid by fermenting a vinasse raw material, and belongs to the technical field of bioengineering. The method comprises the following steps of: dehydrating fresh vinasse and crushing, pretreating to form a fermentation culture medium, pre-hydrolyzing by using enzymes, and saccharifying with fermenting by using enzyme and actinobacillus succinogenes to obtain the succinic acid at the concentration of 25-40g / L, wherein 0.033 to 0.050g of the succinic acid can be produced by using one gram of dry vinasse. The method has the advantages that: the succinic acid is produced by fermenting industrial waste vinasse which is used as a raw material, the vinasse is used as a carbon source instead of glucose, and a nitrogen source is not required tobe externally added in the fermentation culture medium, so that the problem that the petrochemical resource for chemically synthesizing the succinic acid is tense is solved, the environment pollutioncaused by the vinasse is also prevented, and the using value of the vinasse is enhanced.

Description

technical field [0001] The invention relates to a method for producing succinic acid by using industrial waste distiller's grains as raw materials through microbial fermentation, and belongs to the technical field of bioengineering. Background technique [0002] Succinic acid, also known as succinic acid, has the molecular formula C 4 h 6 0 4 , with a molecular weight of 118.09, is an important compound in industry. In the food industry, the main use of succinic acid is as a food flavoring agent, acidulant, neutralizer, pH modifier, flavor substance and antibacterial agent. Succinic acid has been identified as GRAS (generally recognized as safe) by the U.S. FDA; in the pharmaceutical industry, it is used as an important pharmaceutical synthesis intermediate, and is a raw material for the production of many antibiotics, amino acids and vitamins; in the chemical industry, succinic acid The main use of acid is as an ion chelating agent to prevent metal corrosion and pitting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12P19/14C12R1/04
Inventor 郑璞孔德诚
Owner JIANGNAN UNIV
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