Genetic testing kit for infected bacteria in cerebrospinal fluid

Abstract

The invention discloses a genetic testing kit for infected bacteria in cerebrospinal fluid. The genetic testing kit comprises (1) a gene chip and (2) a primer, wherein the gene chip is provided with a. a probe used for detecting 10 bacterial colonies, b. a DNA (Deoxyribonucleic Acid) sequence labeled with biotin points, and c. a DNA sequence with a coding beta-globulin, the probe is SEQ ID Nos: 1-12, the DNA sequence labeled with the biotin points is SEQ ID No.13, and the DNA sequence with the coding beta-globulin is SEQ ID No.14 as an internal locus of control; and the primer includes a first primer used for amplifying 16S rRNA conserved sequences of a majority of common bacteria, a second primer used for amplifying 23S rRNA conserved sequences of the bacteria, and a third primer used for amplifying the internal locus of control, DNA sequences of first primer are SEQ ID Nos: 15-17, DNA sequences of the second primer are SEQ ID Nos.18-19, and DNA sequences of the third primer are SEQ ID Nos.20-21. The genetic testing kit for the infected bacteria in the cerebrospinal fluid, disclosed by the invention, can be used for quickly and accurately detecting 10 common bacteria in the cerebrospinal fluid, and has an important significance in correctly diagnosing and treating an intracranial infector in time.

Description

technical field [0001] The invention relates to the field of genes, in particular to a gene detection kit for bacteria infected with cerebrospinal fluid. Background technique [0002] With the advancement of medical methods, the cases of neurosurgery (traumatic brain injury, brain tumor, cerebral hemorrhage) are increasing day by day. At the same time of surgical treatment, the body's immune system is greatly traumatized during the operation, resulting in a decrease in body immunity, which can easily lead to intracranial infection. In addition, hospital-acquired infection is also an important cause of intracranial infection. Bacterial cerebrospinal meningitis caused by intracranial infection is a common central nervous system disease with acute onset and high fatality rate. If timely and correct diagnosis and treatment can be obtained, most patients will have a good prognosis, and a small number of severe infections may lead to death or sequelae. Therefore, it is very impo...

AI Technical Summary

Problems solved by technology

This method has low sensitivity, long detection cycle, and is easily limited by the physiological conditions of bacteria and the use of antibiotics, and cannot be used for the identification of bacteria or new strains that cannot be cultured at present.

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