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Method for immobilizing enzyme by using magnetic ionic liquid composite material

A magnetic ionic liquid and composite material technology, applied in the field of immobilized enzymes using magnetic ionic liquid composite materials, can solve the problems of easy inactivation of free enzymes, difficulty in continuous use, and poor dispersion, so as to reduce costs, improve utilization rates, and improve Dispersion effect

Inactive Publication Date: 2011-11-23
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional free enzymes have problems such as easy inactivation, poor dispersion, difficult recovery and continuous use in application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of magnetic nanoparticles

[0019] Synthesis of Magnetic Fe by Co-precipitation 3 o 4 nanoparticles. Under nitrogen and fast mechanical stirring conditions, 4.66g FeCl 3 ·6H 2 O and 1.72g FeCl 2 4H 2 O was dissolved in 100 mL of deionized water, and 20 mL of 25% NH 3 ·H 2 O, the reaction system turns black. The system was reacted at 80° C. for 30 min. After the reaction was completed, the magnetic separation was performed, and the supernatant was washed repeatedly with deionized water until the supernatant was neutral.

[0020] 2. Preparation of magnetic ionic liquid composites with immobilized enzymes by adsorption

[0021] 60mmol alkylimidazole and 60mmol chloropropyltriethoxysilane were stirred and reacted at 90°C for 24h. After the reaction finishes, wash repeatedly with diethyl ether and remove diethyl ether by rotary evaporation at room temperature to obtain product 1-alkyl-3-propylethoxysilyl-imidazole chloride salt ([C 1 C(S)Im]Cl, S re...

Embodiment 2

[0027] 1. Preparation of magnetic nanoparticles

[0028]With embodiment 1.

[0029] 2. Preparation of magnetic ionic liquid composites with immobilized enzymes by covalent bonding

[0030] 100mg magnetic nano-Fe 3 o 4 After the particles were ultrasonically dispersed in 100 mL of ethanol for 30 min, 1 g of bromooctanoic acid or bromobutyric acid was added thereto. The system was ultrasonically reacted for 15 min under the protection of nitrogen. After the reaction, the solid particles obtained by magnetic separation were washed twice with 100 mL of acetonitrile, twice with 100 mL of methanol, and dried in vacuum to obtain a solid powder. The obtained solid powder was ultrasonically dispersed in THF, and then 2 g of butyraldehyde-substituted imidazole was added to react at 60° C. under nitrogen protection for 48 h. After the reaction, the solid particles were washed several times with THF to obtain the anions loaded on the magnetic nanoparticles as Cl - of ionic liquids. ...

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Abstract

The invention belongs to the field of enzyme immobilizing, and specifically relates to a method for immobilizing enzymes by using a magnetic ionic liquid composite material. According to the invention, the method comprises the steps that: (1) magnetic nano-particles are prepared; (2) ionic liquid is loaded in the magnetic nano-particles, such that the magnetic ionic liquid composite material is obtained; (3) enzymes are immobilized by using the magnetic ionic liquid composite material. According to the invention, the ionic liquid is loaded in the magnetic nano-carriers, such that a magnetic ionic liquid composite material enzyme immobilizing system is formed. With the method, an ionic liquid microenvironment is introduced, and the ionic liquid has almost no vapor pressure, such that the method has environmental protecting characteristic and designability. With the method, enzyme activity and stability can be improved. According to the invention, a nano-material is used as a carrier in enzyme immobilizing, such that the dispersibility of a catalyst can be improved.

Description

technical field [0001] The invention relates to the field of enzyme immobilization, in particular, the invention relates to a method for immobilizing enzymes using a magnetic ionic liquid composite material. Background technique [0002] As a highly efficient and specific biocatalyst, enzyme plays an important role in the development of industrial chemistry in the 21st century. This is due to the advantages of specificity, mild reaction conditions, low pollution, and high selectivity of the enzyme. Enzymes play a pivotal role in fields closely related to human life, such as medicine, energy, food, and organic synthesis. Traditional free enzymes have problems such as easy inactivation, poor dispersion, difficult recovery and continuous use in application. Enzyme immobilization technology is one of the effective measures to solve these problems. The immobilized enzyme not only retains the catalytic properties of the enzyme, but also can improve the dispersion and operation s...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/02
Inventor 郭晨高红帅江洋洋刘春朝刘会州
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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