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Method for separating and purifying erythritol from yeast fermentation broth

A technology for erythritol and yeast fermentation, applied in the field of bioengineering, can solve the problems of inability to improve the quality of erythritol, and achieve the effects of low cost, convenient operation and high yield

Active Publication Date: 2013-07-17
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some problems in the existing process methods, and the quality improvement of erythritol cannot be realized.

Method used

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  • Method for separating and purifying erythritol from yeast fermentation broth
  • Method for separating and purifying erythritol from yeast fermentation broth
  • Method for separating and purifying erythritol from yeast fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Fermentation broth pretreatment: Centrifuge the erythritol fermentation broth at 4000rpm for 10 minutes to obtain bacteria and supernatant, heat the supernatant to 80°C, keep the temperature for 20min, and set aside;

[0039] (2) Supernatant treatment: The supernatant treated in step (1) is first filtered through a microporous membrane made of polyethersulfone with a pore size of 0.22 μm, and then filtered through an ultrafiltration membrane made of polyethersulfone with a molecular weight cut-off of 3000. Membrane ultrafiltration to obtain filtrate;

[0040] (3) Desalination: The filtrate of step (2) is first passed through a 001×8 strong acidic cation exchange resin column, and then passed through a 201×7 strong basic anion exchange resin column. The anion and cation exchange resins are packed separately, and the height of the resin packed column is 30cm in diameter, 3cm in diameter, and 30°C column temperature, collect the desalted column liquid;

[0041] (4) Co...

Embodiment 2

[0044] (1) Fermentation broth pretreatment: Centrifuge the erythritol fermentation broth at 4500 rpm for 20 minutes to obtain bacteria and supernatant, heat the supernatant to 85°C, keep the temperature for 10 minutes, and set aside;

[0045] (2) Supernatant treatment: The supernatant treated in step (1) is first filtered through a microporous filter membrane made of polytetrafluoroethylene with a pore size of 0.45 μm, and then filtered with a polycarbonate filter with a molecular weight cut-off of 2000. Ultrafiltration membrane ultrafiltration to obtain filtrate;

[0046] (3) Desalination: The filtrate of step (2) is first passed through a 7320 strong acidic cation exchange resin column, and then through a 201×4 strong basic anion exchange resin column. The anion and cation exchange resins are packed separately, and the height of the resin packed column is 35cm , the diameter is 3cm, the column temperature is 35°C, and the desalted column solution is collected;

[0047] (4) ...

Embodiment 3

[0050] (1) Fermentation broth pretreatment: Centrifuge the erythritol fermentation broth at 5000 rpm for 15 minutes to obtain bacteria and supernatant, and heat the supernatant to 90° C., keep the temperature for 15 minutes, and set aside;

[0051] (2) Supernatant treatment: The supernatant treated in step (1) is first filtered through a microporous filter membrane made of polyethersulfone with a pore size of 0.45 μm, and then ultrafiltered with a polyamide material with a molecular weight cut-off of 3500 Membrane ultrafiltration to obtain clarified liquid;

[0052] (3) Desalination: The clarified solution of step (2) is first passed through a 001×7 strong acid cation exchange resin column, and then through a 201×7 strong basic anion exchange resin column. The anion and cation exchange resins are packed separately, and the resin is packed into a column The height is 40cm, the diameter is 3.5cm, the column temperature is 40°C, and the desalted column liquid is collected;

[00...

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Abstract

The invention discloses a method for separating and purifying erythritol from yeast fermentation liquid. The steps of the preparation method include fermentation broth pretreatment, supernatant liquid treatment, desalting, column liquid concentration, crystallization and recrystallization. The method of the present invention uses erythritol yield as an indicator, adopts a static optimization method that combines single factor experiments and orthogonal experiments, optimizes the purification process, has reasonable process conditions, and has good efficacy stability. The invention can solve the current problems of complicated production process of the product, inability to separate a large amount of soluble proteins and macromolecular organic matter in the fermentation broth, and low yield, and has the advantages of mild conditions, short production cycle, white product color, high purity, and ease of use. Industrial production and other advantages.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for separating and purifying erythritol from yeast fermentation liquid. Background technique [0002] Erythritol (1,2,3,4-butanethritol) is a natural four-carbon sugar alcohol. It is widely found in seaweed, mushrooms, melons, grapes, pears and fermented foods; at the same time, it also exists in the tissues and body fluids of humans and animals. It is a white crystalline powder with no peculiar smell and has a sweetness of about 75% of sucrose. [0003] As one of the most popular natural low-calorie sweeteners, erythritol has been widely used in the food industry. Because the heat of dissolution of erythritol is relatively low, it has a strong heat absorption effect when dissolving, and it will produce a refreshing feeling when it is in the mouth. Therefore, when it is used in chocolate, eight-treasure porridge, candy and other foods, it will have a cool and refreshing f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C31/24C07C29/76C07C29/78
Inventor 胡永红杨文革孟小琴
Owner NANJING TECH UNIV
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