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Method for separating and purifying erythritol from yeast fermentation broth

A technology for erythritol and yeast fermentation, which is applied in the field of bioengineering, can solve problems such as the inability to improve the quality of erythritol, and achieve the effects of low cost, mild process conditions, and stable quality

Active Publication Date: 2011-11-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some problems in the existing process methods, and the quality improvement of erythritol cannot be realized.

Method used

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  • Method for separating and purifying erythritol from yeast fermentation broth
  • Method for separating and purifying erythritol from yeast fermentation broth
  • Method for separating and purifying erythritol from yeast fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Fermentation broth pretreatment: Centrifuge the erythritol fermentation broth at 4000rpm for 10 minutes to obtain bacteria and supernatant, heat the supernatant to 80°C, keep the temperature for 20min, and set aside;

[0039] (2) Supernatant treatment: The supernatant treated in step (1) is first filtered through a microporous membrane made of polyethersulfone with a pore size of 0.22 μm, and then filtered through an ultrafiltration membrane made of polyethersulfone with a molecular weight cut-off of 3000. Membrane ultrafiltration to obtain filtrate;

[0040] (3) Desalination: The filtrate of step (2) is first passed through a 001×8 strong acidic cation exchange resin column, and then passed through a 201×7 strong basic anion exchange resin column. The anion and cation exchange resins are packed separately, and the height of the resin packed column is 30cm in diameter, 3cm in diameter, and 30°C column temperature, collect the desalted column liquid;

[0041] (4) Co...

Embodiment 2

[0044] (1) Fermentation broth pretreatment: Centrifuge the erythritol fermentation broth at 4500 rpm for 20 minutes to obtain bacteria and supernatant, heat the supernatant to 85°C, keep the temperature for 10 minutes, and set aside;

[0045] (2) Supernatant treatment: The supernatant treated in step (1) is first filtered through a microporous filter membrane made of polytetrafluoroethylene with a pore size of 0.45 μm, and then filtered with a polycarbonate filter with a molecular weight cut-off of 2000. Ultrafiltration membrane ultrafiltration to obtain filtrate;

[0046] (3) Desalination: The filtrate of step (2) is first passed through a 7320 strong acidic cation exchange resin column, and then through a 201×4 strong basic anion exchange resin column. The anion and cation exchange resins are packed separately, and the height of the resin packed column is 35cm , the diameter is 3cm, the column temperature is 35°C, and the desalted column solution is collected;

[0047] (4) ...

Embodiment 3

[0050] (1) Fermentation broth pretreatment: Centrifuge the erythritol fermentation broth at 5000 rpm for 15 minutes to obtain bacteria and supernatant, and heat the supernatant to 90° C., keep the temperature for 15 minutes, and set aside;

[0051] (2) Supernatant treatment: The supernatant treated in step (1) is first filtered through a microporous filter membrane made of polyethersulfone with a pore size of 0.45 μm, and then ultrafiltered with a polyamide material with a molecular weight cut-off of 3500 Membrane ultrafiltration to obtain clarified liquid;

[0052] (3) Desalination: The clarified solution of step (2) is first passed through a 001×7 strong acid cation exchange resin column, and then through a 201×7 strong basic anion exchange resin column. The anion and cation exchange resins are packed separately, and the resin is packed into a column The height is 40cm, the diameter is 3.5cm, the column temperature is 40°C, and the desalted column liquid is collected;

[00...

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Abstract

The invention discloses a method for separating and purifying erythritol from a yeast fermentation broth. The preparation method provided by the invention comprises the following steps of: pretreatment of a fermentation broth, treatment of a liquid supernatant, desalting, column liquid concentration, crystallizing and re-crystallizing. According to the method provided by the invention, the erythritol yield is taken as an index, a static optimization method combining a single factor experiment and an orthogonal experiment is adopted, so that a purifying process is optimized; and the method hasrational process conditions and good effect stability. The method for separating and purifying the erythritol from the yeast fermentation broth can solve the problems as follows: the traditional manufacturing process of the product is complicated, a lot of soluble proteins and macromolecular organic matters in the fermentation broth cannot be separated, the yield is low and the like; and the method has the advantages of moderate conditions, short production period, white color and luster of the product, high purity, easiness of industrial production and the like.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for separating and purifying erythritol from yeast fermentation liquid. Background technique [0002] Erythritol (1,2,3,4-butanethritol) is a natural four-carbon sugar alcohol. It is widely found in seaweed, mushrooms, melons, grapes, pears and fermented foods; at the same time, it also exists in the tissues and body fluids of humans and animals. It is a white crystalline powder with no peculiar smell and has a sweetness of about 75% of sucrose. [0003] As one of the most popular natural low-calorie sweeteners, erythritol has been widely used in the food industry. Because the heat of dissolution of erythritol is relatively low, it has a strong heat absorption effect when dissolving, and it will produce a refreshing feeling when it is in the mouth. Therefore, when it is used in chocolate, eight-treasure porridge, candy and other foods, it will have a cool and refreshing f...

Claims

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Application Information

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IPC IPC(8): C07C31/24C07C29/76C07C29/78
Inventor 胡永红杨文革孟小琴
Owner NANJING UNIV OF TECH
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