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Linolenic acid-carnosine composite and preparation method thereof

A technology of linolenic acid and complexes, which is applied in drug combinations, anti-toxic agents, metabolic diseases, etc., can solve the problems of reduced bioavailability, unstable physical and chemical properties, and weak ability, so as to improve permeability and biological stability , the effect of inhibiting tumor

Inactive Publication Date: 2011-11-16
南京百吉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, carnosine is a small molecule dipeptide, which is easily degraded by specific carnosine enzymes in the body, and carnosine is a water-soluble substance, which has a weak ability to pass through the natural biofilm barrier, resulting in unstable physical and chemical properties and bioavailability reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Mix 1000 mg of dry carnosine powder with 100 ml of PH11 100 mM borax-sodium carbonate buffer to obtain a reaction solution; (2) Drop 7 ml of linolenic acid chloride into the reaction solution prepared in the first step, and place the resulting solution in The reaction was stirred at 40°C for a period of 30 hours, during which the pH of the reaction solution was adjusted to 11 with NaOH; (3) The pH of the reaction solution treated in the previous step was adjusted to 4 with HCL, and the reaction was terminated. 100mM borax-sodium carbonate buffer to make up the volume of the reaction solution to 150ml to obtain the crude linolenic acid-carnosine complex solution, take 200ul of the crude solution to determine the degree of modification by ninhydrin colorimetry; (4) use the crude product prepared in the previous step The solution was applied to an RT-HPLC chromatography column and eluted with acetone at a flow rate of 1ml / min. The absorption peak was detected by 210nm UV...

Embodiment 2

[0018] (1) Mix 1000 mg of dry carnosine powder with 100 ml of pH11 100 mM borax-sodium carbonate buffer to obtain a reaction solution; (2) Drop 15 ml of linolenic acid chloride into the reaction solution prepared in the first step, and place the resulting solution in The reaction was stirred at 50°C for a period of 36 hours, during which the pH of the reaction solution was adjusted to 11 with NaOH; (3) The pH of the reaction solution treated in the previous step was adjusted to 4 with HCL, and the reaction was terminated. 100mM borax-sodium carbonate buffer to make up the volume of the reaction solution to 150ml to obtain the crude linolenic acid-carnosine complex solution, take 200ul of the crude solution to determine the degree of modification by ninhydrin colorimetry; (4) use the crude product prepared in the previous step The solution was applied to an RT-HPLC chromatography column and eluted with acetone at a flow rate of 1ml / min. The absorption peak was detected by 210nm U...

Embodiment 3

[0020] (1) Mix 1000 mg of carnosine dry powder with 100 ml of PH11 100 mM borax-sodium carbonate buffer to obtain a reaction solution; (2) Drop 8 ml of linolenic acid chloride into the reaction solution prepared in the first step, and place the resulting solution in The reaction was stirred at 20°C for a period of 24 hours, during which the pH of the reaction solution was adjusted to 11 with NaOH; (3) The pH of the reaction solution treated in the previous step was adjusted to 4 with HCL, and the reaction was terminated. 100mM borax-sodium carbonate buffer to make up the volume of the reaction solution to 150ml to obtain the crude linolenic acid-carnosine complex solution, take 200ul of the crude solution to determine the degree of modification by ninhydrin colorimetry; (4) use the crude product prepared in the previous step The solution was applied to an RT-HPLC chromatography column and eluted with acetone at a flow rate of 1ml / min. The absorption peak was detected by 210nm UV...

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Abstract

The invention provides a linolenic acid-carnosine composite. The structural general formula of the composite molecule is His-Ala-C(0)(CH2)6(CH=CHCH2)3CH2CH3. By utilizing the linolenic acid-carnosine composite prepared by the scheme of the invention, the problem that carnosine and linolenic acid are unstable can be effectively solved, linolenic acid has less possibility of losing efficacy caused by oxidization and decomposition; and carnosine has less possibility of being degraded and separated by carnosinase and the biological stability of carnosine can be increased.

Description

Technical field [0001] The invention relates to the technical field of modified complexes of peptides or proteins and their preparation. Specifically, the present invention relates to a fatty acid-carnosine complex and a preparation method thereof. Background technique [0002] Carnosine is a dipeptide composed of β-alanine and histidine. It is mainly found in the skeletal muscle of certain vertebrates including humans, and can be synthesized in animals with a molecular weight of 226.24. Carnosine has anti-oxidant properties and is a natural antioxidant; the transmittance of turbid human eye lenses can be enhanced. Anti-oxidant preparations using carnosine as raw materials can treat senile cataracts. Used in cosmetics, it can prevent skin aging and skin whitening. Carnosine can prevent free radicals generated by smoking. These free radicals can damage the skin more than sunlight. Free radicals are very active in the human body. Strong atoms or groups of atoms can oxidize other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D233/64A61P35/00A61P39/06A61P3/06A61P9/12A61P29/00
Inventor 王沁夏涛
Owner 南京百吉生物科技有限公司
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