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Micro-fluidic cell suspension culture chip and application thereof

A cell suspension, microfluidic technology, applied in tissue cell/virus culture devices, enzymology/microbiology devices, bioreactor/fermenter combinations, etc., can solve the complex and limited chip design and processing and drive control systems The number of units, the area of ​​the chip occupied, etc., to achieve the effect of less cross-contamination, easy control, and low cost

Inactive Publication Date: 2013-02-13
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the number of integrated culture units increases, the number of drive channels and external interfaces used as micropumps will also be increased by three times. A large number of external interfaces and internal pipelines not only make the design and processing of the chip and the drive control system more complicated, but also occupy a large amount of space. A large number of chip areas limit the further improvement of the number of units and cannot meet the needs of future construction of high-throughput cell suspension culture chips

Method used

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  • Micro-fluidic cell suspension culture chip and application thereof
  • Micro-fluidic cell suspension culture chip and application thereof
  • Micro-fluidic cell suspension culture chip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1 refers to Figure 1A ~D, this embodiment is composed of a culture array distributed on a chip, the array includes 4 culture units 100 connected in series on a plane, and each culture unit includes a culture channel 111 on the first layer and a culture channel 111 on the first layer. The cultivation channel 121 of the second layer, the two ends of the two sections of the cultivation channel are connected to each other, forming a closed loop that is viewed as a rectangle when viewed from above. The thickness of the first layer 110 of the chip is 1.5 mm. micron, 3 cm long; the second layer 120 of the chip is 10 micron thick, and the culture channel 121 of the second layer is 100 micron wide, 10 micron deep, and 3 cm long; each culture unit has an independent liquid inlet 113 and a liquid outlet 123, with a diameter of 2 mm, used for the injection and discharge of liquid and culture; the shape and size of each culture unit 100 are consistent, and they are arrang...

Embodiment 2

[0086] Embodiment 2 refers to Figure 2A~D, this embodiment is composed of a culture array distributed on a chip, the array includes 4 culture units 200 connected in series on a plane, each culture unit 200 includes a culture channel 211 on the first layer and The cultivation channel 221 of the second layer, the two ends of the two sections of the cultivation channel are connected to each other, forming a closed loop viewed as a rectangle when viewed from above. 10 microns, 3 cm long; the second layer 220 of the chip is 10 microns thick, and the culture channel 221 of the second layer is 100 microns wide, 10 microns deep, and 3 cm long; each culture unit 200 has an independent liquid inlet 213 and outlet Liquid port 223, diameter is 2 millimeters, is used for the injection and discharge of liquid and culture; The shape and size of each culture unit 200 are all consistent, arrange in a row; The upper part of the channel is the third layer 230, this layer is a A layer of elasti...

Embodiment 3

[0087] Embodiment 3 refers to Figure 3A ~D, this embodiment is composed of a culture array distributed on a chip, the array includes 4 culture units 300 connected in series on a plane, each culture unit 300 includes a culture channel 311 on the first layer, The cultivation channel 321 of the second layer, the cultivation channel 331 of the third layer, the cultivation channel 341 of the fourth layer, and the cultivation channel 351 of the fifth layer, the two ends of these cultivation channels are connected successively, forming a rectangular shape viewed from above. Closed circuit, the thickness of the first layer 310 of the chip is 1.5 mm, the culture channel 311 of the first layer is 100 microns wide, 10 microns deep, and 3 cm long; the second layer 320 of the chip, the third layer 330 of the chip, the fourth layer 340 of the chip, The fifth layer 350 of the chip and the sixth layer 360 of the chip both have a thickness of 10 microns, and the cultivation channel 321 of the...

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Abstract

The invention discloses a micro-fluidic cell suspension culture chip comprising more than one culture unit, wherein each culture unit comprises a substrate, the substrate comprises culture channels which are respectively distributed on multiple laminated culture layers, the culture channels are communicated mutually to form a closed loop, and the closed loop is communicated with an independent liquid inlet and liquid outlet arranged on each substrate; and multiple linear driving channels are also arranged in each substrate, wherein each driving channel intersects with two or more selected culture channels, an elastic diaphragm for isolating each driving channel from each culture channel is arranged in each intersection, and both two ends of each driving channel are communicated with the outer part of each substrate. The chip disclosed by the invention has a simple structure and low cost, is easy to produce and control, has high number density of the culture units, and can be used as asuspension culture container for microbes and biological tissues and as a special biochemical reaction container.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to the structure and application of a microfluidic cell suspension culture chip. Background technique [0002] Microfluidic technology is characterized by a micropipeline network. Micropipes, micropumps, microvalves, microreservoirs, microelectrodes, microdetection elements and other functional components are integrated into chip materials like integrated circuits through microprocessing technology. superior. Microfluidic technology has extremely high efficiency. Due to its small structure, it is easy to integrate dozens or hundreds of micro-organism micro-culture chambers on the chip at a time, achieving high-throughput culture and greatly improving the efficiency of microbial experiments. However, the existing high-throughput microfluidic cell culture chips cannot meet the requirements of microbial culture in industrial fermentation. The most important liquid fermentation method in the fer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/02C12M1/00
CPCC12M23/16C12M21/08C12M23/34
Inventor 陈立桅甘明哲
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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