In vitro cell co-culturing double flow mechanical force loaded flow chamber apparatus

Abstract

The invention provides an in vitro cell co-culture double flow mechanical force loaded flow chamber apparatus which mainly comprises five parts: a screw cover plate, a glass cover plate, a flow chamber pedestal, left / right joints and a flow chamber co-culturing nested block. The screw cover plate is arranged on the glass cover plate to tightly press the glass cover plate. The glass cover plate is arranged above the flow chamber pedestal and sealed with an O-shaped ring to guarantee aseptic atmosphere in the chamber. The upper surface of the flow chamber pedestal is provided with an annular groove for the installation of the O-shaped ring; location holes are provided in the front and back interior walls of the flow chamber pedestal for the positioning of the co-culturing nested block; two sides of the pedestal are provided with fluid passages for communication with a perfusion system. The left / right joints are connected with the chamber through pipe threads. The upper part of the co-culturing nested block is provided with a slide glass embedding groove and a flow chamber groove for the placing of an upper slide glass, and the bottom of the co-culturing nested block is provided with a lower flow chamber groove for the placing of a lower slide glass. According to the invention, the flow chamber apparatus is connected with the perfusion system, cells are in the flow chamber, and released molecules vertically disperse in a nutrient solution and have a small diffusion distance, thereby realizing the purpose of co-culturing.

Description

technical field [0001] The invention relates to the field of biomechanical engineering research, in particular to a flow chamber device capable of co-cultivating two kinds of cells and simultaneously loading the two kinds of cells with shear stress. Background technique [0002] Biomechanics, as an emerging interdisciplinary subject, has attracted more and more attention from the scientific community at home and abroad. As a mechanical loading device, biomechanics has also been widely used in the study of cell mechanics in in vitro flow environments. The initial steady flow flow chamber was designed by Frangos, Eskin et al. (Eskin SG, Ives CL, Mcintire LV, et al. Response of cultured endothelial cells to steady flow[J]. Microvasc Res, 1984, 28: 87-94; Frangos JA, Eskin SG, Mcintire LV, et al.Floweffect on prostacyclin production by cultured human endothelial cues[J].Science, 1985, 227:1477-1479.), in the early studies, the flow chambers used by scientists were This single c...

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Problems solved by technology

[0005] However, the above two flow chambers are somewhat flawed

G.Ed Rainger et al.’s co-culture flow chamber can only apply shear stress to one type of endothelial cells, which has a narrow scope of application, has scientific limitations, and cannot simultaneously load two types of cells; the dynamic culture of Gianni Ciofani et al. The dual-chamber can satisfy the dynamic co-culture of two kinds of cells, but its dual-chamber is mainly designed for the study of biochemical medicine such as pharmacokinetics, molecular biology and drug delivery experiments, and can only achieve low-flow co-culture. The aspect ratio is small, the shear force of the liquid flow is small, and the flow velocity of the bottom layer of the cell culture in the chamber is slow, which cannot meet the requirements of mechanical load on the cells in biomechanics

In addition, all devices cannot be extended

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