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Plantain seed extract and application thereof

A technology of psyllium extract and psyllium, which is applied in the field of traditional Chinese medicine, can solve the problems of liver and kidney toxicity, adverse reactions, side effects, etc., and achieve the effects of low toxicity, promoting consumption, and reducing fasting blood sugar levels

Active Publication Date: 2011-11-02
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These drugs have a certain therapeutic effect on diabetes, but they are also accompanied by many side effects, such as adverse reactions in the digestive tract and certain liver and kidney toxicity.

Method used

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  • Plantain seed extract and application thereof
  • Plantain seed extract and application thereof
  • Plantain seed extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Remove sand and impurities, crush the psyllium seeds into small particles of 4 mm, sieve, reflux extract with 4 times the volume of 75% ethanol for 4 times, filter the extract, recover the solvent to dryness, and suspend with normal saline before use.

[0022] Weigh an appropriate amount of psyllium extract sample, dissolve it in a volumetric flask with a solution of 0.1% (V:V) formic acid aqueous solution and methanol at a volume ratio of 90:10, dilute to the mark, shake well, and make 1mL Solution contains 1g sample, and utilizes ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) to analyze, and analysis condition is as follows:

[0023] Chromatographic conditions: Waters, Acquity UPLC BEH C 18 (50×2.1mm) chromatographic column; the mobile phase A is an aqueous solution containing 0.1% (V:V) formic acid, and the mobile phase B is methanol; the flow rate is 0.3min; the column temperature is 40°C; the injection volume is 2 μL; The detection mode i...

Embodiment 2

[0027] HepG2 cells were inoculated in a 96-well plate, and when the cells grew to 60% confluent, they were replaced with culture medium containing 0.2% bovine serum albumin (BSA) of plantago seed extract at different concentrations, incubated for 24 hours, and detected in the culture medium. The residual amount of glucose, the glucose consumption rate of cells in each well was calculated according to the following formula.

[0028] Glucose consumption rate = (the measured value of the experimental well at 0h-the measured value of the experimental well at 24h) / (the measured value of the control well at 0h-the measured value of the control well at 24h)×100%.

[0029] Table 1 The promoting effect of psyllium extract on the glucose metabolism of HepG2 cells

[0030]

[0031] Note: Metformin is a positive drug. * P** P*** P<0.001, compared to control wells.

[0032] It can be seen from Table 1 that the psyllium extract has a promoting effect on the glucose metabolism of HepG2 ...

Embodiment 3

[0034] At the end of the HepG2 cell glucose consumption test, remove the culture medium to be tested, replace it with a culture medium containing 0.5g / LMTT and continue to incubate for 4h, then pour out the culture medium, blot dry, add 200l dimethyl sulfoxide to each well, mix After uniformity, put it on a microplate reader to measure the absorbance at 570nm, and calculate the survival rate of HepG2 cells according to the following formula: cell survival rate=measured value of experimental wells / measured value of control wells×100%.

[0035] Table 2 Toxicity of psyllium extract to HepG2 cells

[0036]

[0037] Note: Metformin is a positive drug. *** P<0.001, compared to control wells.

[0038] It can be seen from Table 2 that compared with the positive control drug metformin, the plantago extract has less toxicity to HepG2 cells and shows better safety.

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Abstract

The invention discloses a Plantain seed extract and application thereof. The Plantain seed extract is acquired by reflux extraction of Plantain seed with 2-6 volume times of 60-90vol% ethanol aqueous solution 2-6 times. The Plantain seed extract contains acteoside, isoacteoside, geniposidic acid, ferulic acid, and Cheqiansu A and Cheqiansu B. In-vivo experiments show that the Plantain seed extract provided by the invention can promote glucose consumption and has lower toxicity to cells; and simultaneously in-vitro experiments show that the Plantain seed extract can significantly increase serum insulin level and reduce fasting blood-glucose level, presents good medicinal value in treatment of diabetes; therefore, the Plantain seed extract can be used in the preparation of pharmaceutical medicaments for treating diabetes singly or together with a pharmaceutically acceptable composition composed of a drug carrier.

Description

technical field [0001] The invention relates to a plantago seed extract and its application, belonging to the technical field of traditional Chinese medicines. Background technique [0002] At present, diabetes has become one of the three major chronic diseases that seriously endanger the health of modern humans. Diabetes is mainly manifested as "three excesses and one less" symptoms of large appetite, drinking water, excessive urination, and weight loss. At the same time, with the prolongation of the disease time, it can lead to complications in various tissues and organs. Drugs currently used to treat diabetes mainly include sulfonylureas, glinides, biguanides, thiazolidinedione compounds, and α-glucosidase inhibitors. These drugs have a certain therapeutic effect on diabetes, but they are also accompanied by many side effects, such as adverse reactions in the digestive tract and certain liver and kidney toxicity. At the same time, since diabetes is the result of metabol...

Claims

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Application Information

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IPC IPC(8): A61K36/68A61P3/10A61K131/00
Inventor 王峥涛杨莉祁萌季光耿放廖立平
Owner SHANGHAI UNIV OF T C M
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