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Hemolytic agent for blood leukocyte five-classification counting and application thereof

A technology of hemolytic agent and white blood cell, applied in the field of blood analysis, to achieve the effect of strong ability to lyse red blood cells and high stability

Inactive Publication Date: 2013-07-03
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still uses saponin, so there is also the problem of saponin as a hemolytic agent as mentioned above

Method used

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  • Hemolytic agent for blood leukocyte five-classification counting and application thereof
  • Hemolytic agent for blood leukocyte five-classification counting and application thereof
  • Hemolytic agent for blood leukocyte five-classification counting and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Embodiment 1: Preparation of hemolytic agent for counting blood leukocytes with five classifications according to the present invention

[0100]Preparation of hemolytic agent A:

[0101] Accurately weigh 3.0 parts by weight of polyoxyethylene sorbitan monolaurate, 5.0 parts by weight of formic acid, 1.0 parts by weight of tetradecyltrimethylammonium chloride, 5.0 parts by weight of EDTA potassium salt, 1.0 parts by weight of Parts of Sodium Chloride. First add the above tetradecyltrimethylammonium chloride and EDTA potassium salt into the Erlenmeyer flask, then add an appropriate amount of double distilled water at 40°C (water resistance ρ>10MΩ / cm) and stir to dissolve; then add the above Stir the polyoxyethylene sorbitan monolaurate, dissolve and cool to 20°C, add organic acid formic acid to adjust the pH value to 2.90±0.1, and finally add pre-dissolved sodium chloride solution to adjust its osmotic pressure to 60± 5mOsm / Kg.

[0102] Preparation of hemolytic agent B...

Embodiment 2

[0105] Embodiment 2: Preparation of hemolyzing agent for counting blood leukocytes with five classifications according to the present invention

[0106] Preparation of hemolytic agent A:

[0107] Accurately weigh 10.0 parts by weight of polyoxyethylene sorbitan monopalmitate, 1.0 parts by weight of citric acid, 5.0 parts by weight of dodecyltrimethylammonium bromide, 1.0 parts by weight of EDTA potassium salt, 4.0 parts by weight, respectively, with an analytical balance. parts by weight potassium chloride. First add the above-mentioned dodecyltrimethylammonium bromide and EDTA potassium salt in the Erlenmeyer flask, then add an appropriate amount of double-distilled water at 42°C (water resistance ρ>10MΩ / cm) and stir to dissolve it; then add poly Oxyethylene sorbitan monopalmitate was stirred, dissolved and cooled to 24°C, adding organic acid citric acid to adjust the pH value to 4.5±0.1, and finally adding pre-dissolved potassium chloride solution to adjust its osmotic pres...

Embodiment 3

[0111] Embodiment 3: Preparation of hemolytic agent for counting blood leukocytes with five classifications according to the present invention

[0112] Preparation of hemolytic agent A:

[0113] Accurately weigh 8.0 parts by weight of polyoxyethylene sorbitan monostearate, 1.5 parts by weight of acetic acid, 4.1 parts by weight of cetyltrimethylammonium chloride, 1.6 parts by weight of EDTA sodium salt, 3.5 parts by weight, respectively, with an analytical balance. parts by weight sodium chloride. First add the above-mentioned cetyltrimethylammonium chloride and EDTA sodium salt in the Erlenmeyer flask, then add an appropriate amount of double distilled water at a temperature of 50°C (water resistance ρ>10MΩ / cm) and stir to dissolve it; then add poly Oxyethylene sorbitan monostearate was stirred, dissolved and cooled to 25°C, adding organic acid acetic acid to adjust the pH value to 2.0±0.1, and finally adding pre-dissolved sodium sulfate solution to adjust its osmotic pressu...

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Abstract

The invention relates to a hemolytic agent for blood leukocyte five-classification counting and an application thereof. The hemolytic agent comprises a hemolytic agent A and a hemolytic agent B. The hemolytic agent A comprises a polyoxyethylene nonionic surfactant, an organic acid, a quaternary ammonium salt cationic surfactant cosolvent, a stabilizing agent and an osmotic regulator; the hemolytic agent B comprises alkaline inorganic salt and an osmotic regulator. When compared with the result detected by a standard hemolytic agent produced by BeckmanCoulter company, the hemolytic agent of the invention has good correlation and high accuracy. Especially, when abnormal cells exist in a blood specimen, the hemolytic agent has high warning sensitivity and good specificity, and exhibits the most beneficial effect.

Description

【Technical field】 [0001] The invention belongs to the technical field of blood analysis. More specifically, the present invention relates to a hemolytic agent for counting white blood cells in five different categories, and also relates to the use of the hemolytic agent. 【Background technique】 [0002] In clinical hematology tests, counting and classifying peripheral blood leukocytes are of great significance to the diagnosis and treatment of many diseases. Leukocyte differentiation using automated blood analysis equipment usually requires the use of hemolytic agents. On the one hand, the hemolytic agent can dissolve red blood cells and release the hemoglobin in the cells for the determination of hemoglobin. On the other hand, hemolytic agents can eliminate the interference of red blood cells and red blood cell fragments on white blood cell analysis after destroying red blood cells, so that the subsequent white blood cell count and classification can be performed. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49
Inventor 乐家新
Owner GENERAL HOSPITAL OF PLA
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