Method for extracting and separating viterxin and isovitexin from natural product

Abstract

The invention discloses a method for extracting and separating viterxin and isovitexin from a natural product. The method comprises the following steps: based on santalum album L leaves as raw materials, carrying out ultrasonic extraction and lixiviation on the santalum album L leaves by utilizing an alcohol solvent; then respectively extracting santalum album L leaf extract by adopting petroleum ether, chloroform and ethyl acetate in turn; carrying out column chromatography on ethyl acetate extract, and recrystallizing the obtained eluent so as to obtain the isovitexin; and separating a residual liquid through a preparation liquid phase so as to obtain the viterxin. In the method, the santalum album L leaves are used for the first time so as to extract out the viterxin and isovitexin, the separation process is simple, the cost is low, and the raw materials are available; the santalum album L leaves contain high viterxin and isovitexin contents, thus a novel way for resource utilization of santalum album L leaves is provided; the two compounds can be prepared into small hydro-acupuncture drop injections, freeze-dried powder, tablets, capsules or pills; and the viterxin can be used for preparation of medicaments for treating coronary disease, myocardial infarction, angina and cancer prevention tumour resistance, and the isovitexin can be used for preparation of medicaments for treating or preventing prostate cancer, breast cancer and endometrial cancer.

Description

technical field [0001] The invention relates to the field of natural product extraction, in particular to a method for extracting and separating vitexin and isovitexin from natural products. Background technique [0002] sandalwood( Santalum album L ), also known as true sandalwood, white sandalwood, Zhentan, etc., is a semi-parasitic evergreen small tree of the genus Sandalwood in the family Sandalaceae. Native to southern India, Indonesia and other places, it is an important economic tree species integrating medicine, spices, Buddhist supplies, fine handicrafts and excellent carving materials. my country has a long history of using sandalwood. It is recorded in "Compendium of Materia Medica" by Li Shizhen in the Ming Dynasty: "Sandalwood; interpretation name: Zhantan, real sandalwood; smell: warm, pungent, non-toxic; indications: eliminating wind, heat, swelling, poison, and evil in the middle. Ghost qi, killing insects, relieving abdominal pain, cholera, kidney qi pain; ...

AI Technical Summary

Problems solved by technology

The extraction and separation of vitexin and isovitexin from natural plants is generally performed by silica gel column chromatography, SephadexLH-20 column chromatography and preparative HPLC, and the process is relatively complicated.

[0006] According to literature search, there is currently no report about vitexin and isovitexin isolated from sandalwood leaves at home and abroad.

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