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Preparation method of squid skin protein active peptide

A technology of protein activity and squid skin, applied in fish protein components, animal protein processing, protein food components, etc., can solve the problems of increasing protein resource development costs, high prices, and limited application range, so as to protect the liver and reduce costs. Low, immune-boosting effect

Active Publication Date: 2011-10-19
江苏盐综产业投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of general commercial proteases is relatively expensive. When active peptides are prepared by enzymatic methods, the development cost of protein resources is greatly increased, and its application range is limited.

Method used

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  • Preparation method of squid skin protein active peptide
  • Preparation method of squid skin protein active peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Take 1KG squid skin raw materials: wash, drain, and cut into pieces.

[0013] 2. Decolorization treatment: add 4KG 0.045mol / L NaOH solution, soak for 2 days, and change the solution every 12 hours.

[0014] 3. Washing and neutralization: wash with water repeatedly until the pH is neutral.

[0015] 4. Color fixing treatment: add 4KG of Ca(OH) with a mass concentration of 0.4% 2 The solution is a color-fixing agent to fix the pigment protein remaining in the fish skin, soak for 2 days, and change the solution every 12 hours.

[0016] 5. Washing and neutralization: wash with water repeatedly until the pH is neutral.

[0017] 6. Protein extraction: Use pH neutral water as protein extractant, add 4KG water, and treat at 75°C for 0.5h.

[0018] 7. Filtration: remove insoluble protein by filtration to obtain fish skin protein solution.

[0019] 8. High-pressure treatment: Treat the filtered protein liquid at 121°C with high-pressure hot water for 30 minutes to obtain th...

Embodiment 2

[0021] 1. Take 1KG squid skin raw materials: wash, drain, and cut into blocks.

[0022] 2. Decolorization treatment: add 10KG 0.01mol / L NaOH solution as a decolorizer, soak for 8 days, and change the solution every 24 hours.

[0023] 3. Washing and neutralization: wash with water repeatedly until the pH is neutral.

[0024] 4. Color fixing treatment: add 10KG of Ca(OH) with a mass concentration of 0.1% 2 The solution is a color-fixing agent to fix the pigment protein remaining in the fish skin, soak for 4 days, and change the solution every 24 hours.

[0025] 5. Washing and neutralization: wash with water repeatedly until the pH is neutral.

[0026] 6. Protein extraction: Use pH neutral water as protein extractant, add 10KG water, and treat at 55°C for 1.5h.

[0027] 7. Filtration: filter to remove insoluble protein.

[0028] 8. High-pressure treatment: The filtered protein solution was subjected to high-pressure treatment at 110°C for 120 minutes to obtain the squid skin ...

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Abstract

The invention discloses a preparation method of a squid skin protein active peptide, which comprises the following steps: cleaning squid skin, cutting into blocks, adding NaOH water solution, soaking for 2-8 days for decolorization and further washing with water till the pH is neutral; adding CaOH2 solution, soaking for 2-4 days for color fixation, further washing with the water till the pH is neutral; processing for 0.5-1.5 hours in the water with the neutral pH at the temperature of 55-75 DEG C for filtration, removing insoluble proteins and extracting for getting squid skin protein solution; and finally performing high-pressure processing on the solution for 30-120 minutes at the temperature of 110-121 DEG C for getting the squid skin protein active peptide. The squid skin is processed by the dual-alkali process, the decolorization is firstly performed and then the color fixation is performed; the high-pressure processing method is used for processing squid skin proteins for preparing the biological active peptide, the preparation process is simple, the cost is low and the applicability is strong; and the prepared active peptide has the effects of oxidation prevention, senility resistance, liver protection, enhancement of immunity and the like.

Description

technical field [0001] The invention relates to deep-processing products of squid skin protein, provides a preparation method of squid skin protein active peptide, and belongs to the technical field of practical protein preparation. Background technique [0002] Squid is a mollusk cephalopod marine animal. With the development of my country's ocean-going squid fishing industry, squid has become the main raw material for aquatic product processing in my country. According to reports, the annual processing volume of squid in my country is about 300,000 to 400,000 tons, and the squid produced in it The skin accounts for 8-13% of the processed amount, and the protein content in the dry weight of the squid skin is as high as 70-80%. Most factories adopt the method of burying and discarding squid skins, which causes waste of squid protein resources and environmental pollution. Therefore, the development of protein in squid skin is one of the effective ways to increase the economic...

Claims

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Application Information

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IPC IPC(8): A23J1/04A23J3/04A23J3/26
Inventor 方旭波陈小娥余辉陈静谢宁宁
Owner 江苏盐综产业投资发展有限公司
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