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Method for making human pelvic inflammation simulating animal model

An animal model and technology of pelvic inflammatory disease, which is applied in the field of animal model simulation of human pelvic inflammatory disease, can solve the problems that hinder the application of the model and the difficulty of surgery, etc., and achieve the effect of simple operation method, easy method and high success rate

Inactive Publication Date: 2011-10-05
唐和斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The above two methods are different from the occurrence of clinical pelvic inflammatory disease, and the operation is more difficult, which hinders the application of the model

Method used

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  • Method for making human pelvic inflammation simulating animal model
  • Method for making human pelvic inflammation simulating animal model
  • Method for making human pelvic inflammation simulating animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Strain preparation: Escherichia coli was selected, inoculated on beef extract agar medium, and cultured at 37°C for 2 days.

[0043] Animal preparation: Wistar rats without reproductive tract diseases were selected, and 10 animals were selected for each strain.

[0044] Animal inoculation: the rats were inoculated with Escherichia coli in the vagina of the rats using a syringe under strict aseptic conditions for 5 weeks, twice a week.

[0045] Post-inoculation treatment and observation: 5 weeks after the first inoculation, kill the animal, and remove the reproductive organs, such as: vagina, uterus, fallopian tube, ovary, fully rinse with sterile saline, fix for 48 hours and use for disease Physical examination.

[0046]Through observation and analysis, it is found that this method has the following advantages:

[0047] 1. The success rate of the model is as high as 90%. On the third day after vaccination, the genitals are found to be red and swollen, with increased s...

Embodiment 2

[0051] SD rats without reproductive tract diseases were selected, and the rats were treated with estradiol for 1 week before inoculation, 3 times a week. Escherichia coli was selected, inoculated in beef extract agar medium, and cultured at 37°C for 3 days; rats were inoculated with Escherichia coli in the vagina of rats with a syringe under strict aseptic conditions for 4 weeks, twice a week. Four weeks after the first inoculation, the rats were killed, and the reproductive organs, such as: vagina, uterus, fallopian tube, and ovary, were fully rinsed with sterile saline, fixed for 48 hours, and used for pathological examination.

[0052] The results showed: 1. The success rate of the model reached 100%. On the third day after inoculation, the genitals were found to be red and swollen, and the secretions increased (such as figure 1 . Coli A). According to anatomical findings, vaginal swelling, uterine congestion and effusion, cysts in the ovaries with significantly increased ...

Embodiment 3

[0054] Wistar rats without reproductive tract diseases were selected, and the rats were treated with estradiol for 3 days before inoculation, once a day. Staphylococcus aureus was selected, inoculated in beef extract agar medium, and cultured at 37°C for 2 days; rats were inoculated with Escherichia coli in the vagina of the animal with a syringe under strict aseptic conditions for 3 weeks, once a week. Three weeks after the first inoculation, the rats were killed, and the reproductive organs, such as: vagina, uterus, oviduct, and ovary, were fully rinsed with sterile saline, fixed for 48 hours, and used for pathological examination.

[0055] The results showed that the success rate of the model reached 100%. On the third day after inoculation, the vulva was found to be red and swollen, and the secretions increased (such as image 3 . Aureus A). According to anatomical findings, vaginal swelling, uterine congestion and effusion, cysts in the ovaries with significantly increas...

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Abstract

The invention relates to a method for making a human pelvic inflammation simulating animal model. The method comprises the following steps of: selecting a muridae animal with no genital tract disease; and inoculating enterobacteria, staphylococcus or mycotoruloides into an animal vagina under a sterile condition for 1-8 weeks, 1-7 times every week. The method has the advantages of: high model making success rate up to 100 percent, independence of an immune-depressant, remarkable increase in secreta at a vaginal orifice, observation of bacteria through vaginal smear microscopy, observation of vacuole at an ostium uteri, metremia abscess and a large quantity of inflammatory cells in a fallopian tube through section examination of tissues and organs and remarkable increase in neutrophilic granulocytes as proved by blood examination. The method is easy to operate and grasp, has readily-available materials, has no special technical requirement for a user, is accordant with the clinical natural abiogenesis process of pelvic inflammation, and is suitable for researching the occurrence and the development of pelvic inflammation and the mechanism thereof and screening relevant medicaments.

Description

technical field [0001] The invention relates to a method for making an animal model of simulating human pelvic inflammatory disease. Background technique [0002] Pelvic inflammatory disease refers to the inflammation of female internal reproductive organs and surrounding connective tissue and pelvic peritoneum. It includes endometritis, uterine myositis, salpingitis, ovarian inflammation, etc. Pelvic inflammatory disease is a common and refractory disease in gynecology. Severe cases can cause sepsis and sepsis. If not controlled in time, septic shock and even death may occur. It may also lead to menstrual disorders and even infertility. Its incidence is increasing. Clinical studies have shown that the common pathogenic factors of pelvic inflammatory disease are bacteria (including aerobic bacteria and anaerobic bacteria), as well as viruses, molds, mycoplasma, chlamydia, etc. At present, the animal model of pelvic inflammatory disease does not match the mechanism of h...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/565A61K31/573A61K49/00
Inventor 刘敏李玉桑唐和斌杨祥良
Owner 唐和斌
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