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Method for producing ribonucleic acid by high-density fermentation high-nuclear Candida lipolytica

A high-density fermentation and ribonucleic acid technology, applied in the field of ribonucleic acid production, can solve problems such as high energy consumption, and achieve the effects of simplifying production procedures, reducing emissions, and reducing labor intensity

Active Publication Date: 2011-09-21
NANTONG QIUZHIYOU BIOSCI & BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the highest concentration of bacteria in the domestic production of nucleotide fermentation process can only reach 23g / L, and the energy consumption is relatively large

Method used

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  • Method for producing ribonucleic acid by high-density fermentation high-nuclear Candida lipolytica
  • Method for producing ribonucleic acid by high-density fermentation high-nuclear Candida lipolytica
  • Method for producing ribonucleic acid by high-density fermentation high-nuclear Candida lipolytica

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Experimental program
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Effect test

Embodiment 1

[0029] Medium composition

[0030] Slant medium: 30g / L glucose, 3g / L beef extract, 3g / L yeast extract, 5g / L ammonium sulfate, 0.1g / L anhydrous ferrous sulfate and 25g / L agar;

[0031] Shake flask medium: 35g / L molasses, 1.0g / L magnesium sulfate, 1.0g / L urea, 0.1g / L ammonium sulfate, 0.05g / L zinc sulfate, 0.05g / L ferrous sulfate, 0.05g / L of NaCl and 1ml / L of phosphoric acid

[0032] Batch medium: 10g / L molasses, 1.0g / L magnesium sulfate, 1.0g / L urea, 0.1g / L ammonium sulfate, 0.05g / L zinc sulfate, 0.05g / L ferrous sulfate, 0.05 g / L of NaCl and 1ml / L of phosphoric acid

[0033] Feed medium a: molasses of 250g / L;

[0034] Feed medium b: 250g / L molasses, 7g / L magnesium sulfate, 7g / L urea, 2g / L ammonium sulfate, 1g / L zinc sulfate, 1g / L ferrous sulfate, 0.5g / L NaCl and 10ml phosphoric acid;

[0035] High-density continuous fermentation medium: 70g / L molasses, 1.8g / L magnesium sulfate, 1.8 urea, 0.425g / L ammonium sulfate, 0.225g / L ferrous sulfate, 0.225g / L zinc sulfate, 0.075 High-...

Embodiment 2

[0043] culture medium

[0044] Incline medium: 30g / L glucose, 3g / L beef extract, 3g / L yeast extract, 5g / L ammonium sulfate, 0.1g / L anhydrous ferrous sulfate and 25g / L agar

[0045] Shake flask medium: 45g / L molasses, 1.4g / L magnesium sulfate, 1.4g / L urea, 0.5g / L ammonium sulfate, 0.3g / L zinc sulfate, 0.3g / L ferrous sulfate, 0.2g / L of NaCl and 3ml / L of phosphoric acid

[0046] Feed medium a: molasses of 260g / L;

[0047] Feed medium b: 260g / L molasses, 8g / L magnesium sulfate, 8g / L urea, 3g / L ammonium sulfate, 2g / L zinc sulfate, 2g / L ferrous sulfate, 1g / L NaCl and 15ml phosphoric acid

[0048] Batch fermentation medium: 20g / L molasses, 1.4g / L magnesium sulfate, 1.4g / L urea, 0.5g / L ammonium sulfate, 0.3g / L zinc sulfate, 0.3g / L ferrous sulfate, 0.2g / L of NaCl and 3ml / L of phosphoric acid

[0049] Continuous high-density fermentation medium: 80g / L molasses, 2.4g / L magnesium sulfate, 2.4 urea, 0.625g / L ammonium sulfate, 0.312g / L ferrous sulfate, 0.312g / L zinc sulfate, 0.275 g / L...

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Abstract

The invention relates to the technical field of microbes, in particular to method for producing ribonucleic acid by high-density continuous culture of high-nucleic-acid-yield Candida lipolytica in a stirring tank, which is characterized in that by comprising: activating a strain with a collection number of CGMCC No.3558 in a slant culture medium and culturing the strain in a shake flask; and fermenting the strain obtained in the shake flask in the stirring fermentation tank and extracting and separating to obtain ribonucleic acid, wherein in the stirring fermentation tank, intermittent fermentation is performed in a fermentation medium first, high density fermentation culture is performed 3 to 8 hours later under the conditions of constantly adding a supplemented medium a and a supplemented medium b, and when the concentration of dry bacteria reaches 33 to 36g / L, a high-density continuous fermentation culture medium is added batch to perform high-density continuous fermentation. The method improves the bacteria and RNA yield in unit fermentation tank volume greatly, lowers energy consumption and reduces discharge. The process lowers labor intensity and simplifies a production process.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for producing ribonucleic acid of Candida lipolytica with high density and continuous culture in a stirred tank for high-yield nucleic acid. Background technique [0002] RNA is a very important biological macromolecule. It not only plays a key role in gene expression and protein biosynthesis, but also promotes many physiological functions of cells. In recent years, some studies have shown that RNA and its enzymatic hydrolyzate nucleotides have various physiological functions such as maintaining the body's immune function, anti-oxidation, improving the bioavailability of body protein and iron, lowering cholesterol, and anti-aging. [0003] The preparation of RNA by yeast fermentation is the main method of RNA industrial production at present. Therefore, the mutagenesis screening of highly productive nucleic acid strains is the basis for industrial production. Sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/34C12N1/16C12R1/74
Inventor 曹静林忠周长林邱蔚然邱志云陆长德
Owner NANTONG QIUZHIYOU BIOSCI & BIOTECH
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