Construction method of animal model of chronic inflammation

Abstract

The invention belongs to the fields of medical evaluation, detection technology and experimental zoology, and specifically relates to a construction method of animal model of rat which is used for screening medicaments for treating chronic inflammation, and evaluating methods for treating systematic chronic inflammation. The method comprises the following step that healthy male SD rats are administered with lipopolysaccharide by tail intravenous injection, on an every first day of a week for 4-8 weeks persistently, at a dosage of 120-300 [mu]g per kg of body weight, so that pathologic changes, which meet pathological characteristics of chronic inflammation, mainly of an increased ratio of peripheral blood leucocyte count to neutrophile granulocyte, an increased serum hypersensitive C-creative protein level, infiltration of heart, liver, lung, kidney in inflammatory cells, and haemostasis and oedema of tissues are generated. The method has a good repeatability, and operation of the method is simple, easily controllable and mechanism-clear, besides indexes can be analyzed quantitatively and are convenient for statistics treatments. In addition, the constructed animal model is more ideal and more conform to clinic pathological and physiological changes of the type of disease.

Description

technical field [0001] The invention relates to the fields of medical evaluation, detection technology and experimental animal technology, in particular, relates to a method for establishing an animal model of chronic inflammation. Background technique [0002] Inflammation is one of the most important basic pathological processes, and various inflammatory diseases occupy an extremely important position in the spectrum of human diseases. Typical inflammatory diseases, such as chronic bronchitis, are still common diseases that seriously plague the vast majority of people today. Not only that, recent studies have shown that many diseases that were originally thought to have nothing or little to do with inflammation are actually closely related to inflammation. Inflammation is also a pervasive and important factor in the pathogenesis of malignancies that, like cardiovascular disease and diabetes, pose a serious threat to life expectancy. Based on growing evidence similar to t...

AI Technical Summary

Problems solved by technology

After the dose used for modeling was reduced to 0.5mg / kg to 3mg / kg, the inflammatory response induced by LPS was still severe, and as time went on, the death of animals still existed, which affected the conduct of experimental research. Undoubtedly unfavorable for experimental studies of chronic inflammation

If you want to avoid animal death, reduce the consumption of experimental animals, and enhance the controllability of LPS-related chronic inflammation experimental research, it may be feasible to reduce the dose of LPS used in modeling and to intervene in stages, but the specific reduction can still be obtained. Typical chronic inflammatory changes, there is no precedent to follow

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