Method for producing high-energy high-protein feed by fermenting and decomposing polysaccharide in corn steep liquor
A high-protein feed, fermentation and decomposition technology is applied in the field of fermenting and decomposing polysaccharides in corn steep liquor to produce high-energy and high-protein feed, which can solve the problems of heavy burden on the kidneys, protein cannot be completely absorbed and affected, and achieves the improvement of digestion utilization rate, increase Bran utilization, good decomposition effect
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Embodiment 1
[0033] The method for fermenting and decomposing polysaccharides in corn steep liquor to produce high-energy and high-protein feed is characterized in that: the preparation steps are:
[0034] Step 1. Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae were respectively cultured on plate culture medium, the culture temperature was 35°C, and the culture time was 64 hours;
[0035] Step 2, the Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae cultivated in step 1 are respectively expanded and cultivated with Erlenmeyer flask culture medium, the culture temperature is 35 ℃, and the aerobic fermentation is 64 hours;
[0036] Step 3: Add the Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae grown in step 2 into the seed tank, the composition and weight ratio of the medium in the seed tank are: 5% sucrose, 5% corn molasses and 90% water , the ratio of the amount of each strain added to the weight...
Embodiment 2
[0043] The method for fermenting and decomposing polysaccharides in corn steep liquor to produce high-energy and high-protein feed is characterized in that: the preparation steps are:
[0044] Step 1, first culture Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae with plate culture medium respectively, the culture temperature is 33 ℃, and the culture time is 72 hours;
[0045] Step 2, the Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae cultivated in step 1 are respectively expanded and cultivated with Erlenmeyer flask culture medium, the culture temperature is 33 ℃, and the aerobic fermentation is 72 hours;
[0046] Step 3, adding the Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae grown in step 2 into the seed tank, the composition and weight ratio of the culture medium in the seed tank are: 4% sucrose, 4% corn molasses and 92% water , the ratio of the amount of each strain added to...
Embodiment 3
[0053] The method for fermenting and decomposing polysaccharides in corn steep liquor to produce high-energy and high-protein feed is characterized in that: the preparation steps are:
[0054] Step 1, first culture Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae with plate culture medium respectively, the culture temperature is 36 ℃, and the culture time is 48 hours;
[0055] Step 2, the Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae cultivated in step 1 are respectively expanded and cultivated with Erlenmeyer flask culture medium, the culture temperature is 36 ℃, and the aerobic fermentation is 48 hours;
[0056] Step 3, adding the Bacillus subtilis, Bacillus licheniformis, saccharomyces and Aspergillus oryzae grown in step 2 into the seed tank, the composition and weight ratio of the medium in the seed tank are: 6% sucrose, 6% corn molasses and 88% water , the ratio of the amount of each strain added to the wei...
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