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Formaldehyde-free fixative for tissue sample

A tissue specimen and fixative technology, applied in the field of pathology experiments, can solve the problems of shrinkage and hardening and poor preservation of nucleic acid molecules, and achieve the effect of eliminating cover, avoiding toxic effects, and inhibiting shrinkage and hardening.

Inactive Publication Date: 2011-09-07
HARBIN GREEN SPECIMEN TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, shrinkage and hardening will occur in the hinge reaction, and the antigen molecular structure, enzymes, and nucleic acid molecules of tissue cells are not well preserved.

Method used

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  • Formaldehyde-free fixative for tissue sample
  • Formaldehyde-free fixative for tissue sample
  • Formaldehyde-free fixative for tissue sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 95% ethanol ---------------------------------750ml

[0025] Glacial acetic acid ---------------------------------------30ml

[0026] Acetone -------------------------------------- 20ml

[0027] Diethylene glycol ------------------------------------50ml

[0028] Trichloroacetic acid (2%)-----------------------------50ml

[0029] Octyl-decyl-dimethyl ammonium chloride (1%)---50ml

[0030] 3-methoxy-3-methylbutanol (1%)--------50ml

[0031] The above components are miscible at room temperature.

Embodiment 2

[0033] 90% ethanol ------------------------------710ml

[0034] Glacial acetic acid --------------------------------- 20ml

[0035] Acetone ----------------------------------- 30ml

[0036] Diethylene glycol ---------------------------------60ml

[0037] Trichloroacetic acid (2%)--------------------------60ml

[0038] Dioctyldimethylammonium chloride (0.5%)---60ml

[0039] 3-methoxy-3-methylbutanol (1%)---------60ml

[0040] The above components are miscible at room temperature.

Embodiment 3

[0042] 75% ethanol ------------------------------790ml

[0043] Glacial acetic acid --------------------------------- 40ml

[0044] Acetone ------------------------------------ 10ml

[0045] Polyethylene glycol -------------------------------- 40ml

[0046] Trichloroacetic acid (2%)--------------------------40ml

[0047] Didecyldimethylammonium chloride (2%)------40ml

[0048] 3-methoxy-3-methylbutanol (0.5%)------40ml

[0049] The above components are miscible at room temperature.

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Abstract

The invention provides a formaldehyde-free fixative for a tissue sample, which comprises the following ingredients in parts by volume: 710-790 parts of ethanol, 20-40 parts of glacial acetic acid, 10-30 parts of acetone, 40-60 parts of polyhydric alcohol, 40-60 parts of trichloroacetic acid (TDA), 40-60 parts of surface active agent and 40-60 parts of solubilizing agent. The formaldehyde-free fixative is a nontoxic and innocuous chemical reagent; after being proved by cellular morphological experiments, immunohistochemical experiments, molecular pathological experiments and nucleic acid extraction experiments, the formaldehyde-free fixative has relatively comprehensive fixing effect, can replace the formaldehyde and be used in related pathological experiments, thereby fixing the tissue sample.

Description

technical field [0001] The invention relates to in vitro diagnostic reagents, in particular to an environment-friendly formaldehyde-free tissue specimen fixative and its application, belonging to the technical field of pathological experiments. Background technique [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, the pathological specimens of surgical resection and various biopsy tissues are all histopathologically diagnosed by the hospital pathology department on the surgical tissue samples. [0003] Conventional paraffin-embedded HE staining is the most basic technique currently used to process tissue samples, it is the basis of pathological diagnostic methodology, and it is also the reference standard for evaluating the advantages and disadvantages of many new methods and technologies. The traditional method is to place the tissue sample in the tank of the treatment solution, pour or transfer in different treatment solutions in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 李明王德田董建强
Owner HARBIN GREEN SPECIMEN TECH DEV
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