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Cotton leafworm genetically engineered virus I and construction method thereof

A technology of Spodoptera litura and genetic engineering, which is applied in the field of microorganisms to achieve the effects of not easy drug resistance, fast insecticidal speed, and good field application effect.

Inactive Publication Date: 2011-09-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there have been no reports on the successful construction of the genetically engineered virus of Spodoptera litura nucleopolyhedron at home and abroad

Method used

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  • Cotton leafworm genetically engineered virus I and construction method thereof
  • Cotton leafworm genetically engineered virus I and construction method thereof
  • Cotton leafworm genetically engineered virus I and construction method thereof

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Embodiment 1

[0019] The present invention, through genetic engineering method, in wild virus with strong virulence ( Spodoptera litur anucleopolyhedrovirus , SpltMNPV , GenBank accession number: NC-011616 ), on the basis of deletion and recombination, a genetic engineering virus of Spodoptera litura was provided, and a recombinant Spodoptera litura nuclear polyhedrosis virus was constructed, i.e. Spodoptera litura genetic engineering virus No. 1 ( SpltMNPV-△ egt - egfp ) CCTCC NO: V201027. See attached figure 1 , it is a kind of Spodoptera litura genetically engineered virus provided in this embodiment, namely the structural representation of No. 1 Spodoptera litura genetically engineered virus; Among the figure, egt is the ecdysone uridine diphosphate glucosyltransferase gene; P pH is the polyhedrin gene promoter; egfp is the enhanced green fluorescent protein gene. Depend on figure 1 It can be seen that the deletion in its genome egt The gene, ecdysone uridine diphospha...

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Abstract

The invention relates to the technical field of microbes, in particular relating to a genetically engineered virus I (SpltMNPV-Delta egt-egfp) capable of preventing and controlling farming and forestry pests such as cotton leafworm. The virus is preserved in the China Center for Type Culture Collection (CCTCC) with the collection number of CCTCC NO: V201027. In the invention, a genetic engineering method is utilized to delete and recombine the wild virus so as to obtain the cotton leafworm genetically engineered virus. The genome of the virus loses an egt gene, namely an ecdysteroid uridine diphosphate glucosyltransferase gene; and a marker gene, namely an enhanced green fluorescent protein (egfp) gene is inserted to the locus losing the egt gene, wherein the marker gene is controlled by the wild virus polyhedrin (ph) promoter. Compared with the wild virus, the genetically engineered virus has the advantages of higher pesticidal speed, better field application effect and the like.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a genetic engineering virus of Spodoptera litura which can prevent and control agricultural and forestry pests such as Spodoptera litura, i.e. Spodoptera litura Genetic Engineering Virus No. 1 (SpltMNPV-△ egt - egfp ), the taxonomic name is "Spodoptera litura nuclear polyhedrosis virus genetic engineering strain No. 1" (Genetically modified Spodoptera litur a nucleopolyhedrovirus No.1). The virus was preserved by the China Center for Type Culture Collection (CCTCC), the preservation date is December 15, 2010, and the preservation number is CCTCC NO:V201027. Background technique [0002] Spodoptera litura is one of the main pests of crops, and it is distributed in various provinces and regions in China. The insectivorous nature is extremely wide, and the host plants known to be harmful are more than 290 kinds in 99 families. In recent years, with the change of agricultural ...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/85A01P7/04C12R1/93
Inventor 朱江浦冠勤郭大磊王文兵汤欣欣孙兴鲁秦启联
Owner SUZHOU UNIV
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