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Microbial bacterial agent for degrading straws

A microbial bacterial agent and bacterial agent technology, applied in the field of microbial bacterial agent that efficiently degrades straw, to achieve the effects of increasing soil humus, improving the effect, and improving soil fertility

Inactive Publication Date: 2013-02-27
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The genus Bacillus is an important group of microorganisms with strong cellulose degrading ability. The research on their degradation of straw fiber lignin has been reported, but Bacillus licheniformis as a microbial agent for efficiently degrading straw and its straw degradation effect are rarely reported. to report

Method used

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  • Microbial bacterial agent for degrading straws
  • Microbial bacterial agent for degrading straws
  • Microbial bacterial agent for degrading straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of Microbial Inoculant for Efficiently Degrading Straw

[0043] (1) Fermentation tank culture solution formula: beef juice 0.5%, peptone 0.5%, NaCl 0.5%, soybean oil 0.5%, glucose 0.5%, magnesium sulfate 0.08%, potassium dihydrogen phosphate 0.04%, calcium carbonate 0.5%, 800 μM Cu , Zn, Mn, Fe, stirring. The pH value before sterilization was 7.6, and the pH value after sterilization was 7.4.

[0044] (2) Training process

[0045] Streak inoculation of Bacillus licheniformis JLC38 and Bacillus Weidenstetten JLC43 strains on beef juice peptone medium respectively, culture at 28-30°C for 48 hours, then transfer to 500mL Erlenmeyer flasks respectively, at 30°C, 180r / min , cultivated for 18h. Then insert in the 15L seed tank by 1% inoculum amount, at 180r / min, pH7.5, under the ventilation rate 0.5vvm, cultivate after 24h, then pack in the fermentor of 100L by 5% inoculum amount, in 200r / min, pH 7.5, ventilation 0.6vvm, cultivated for 3 days.

[00...

experiment example 1

[0047] Experimental example 1 In situ ground cover microbial degradation experiment of wheat straw

[0048] Combination experiments of straw decomposing bacteria agents include: A1: Bacillus Weidenstetten C15 + Bacillus Weidenstetten C17; A2: Bacillus Weidenstetten C26 + Bacillus Weidenstetten C29; Denstetten Bacillus JLC43+Bacillus licheniformis JLC38; CK1 (without urea); CK2 (with urea).

[0049] The experiment was carried out under field conditions, and the area of ​​each plot was 20m 2 . When the corn plant height is 30-40cm, spread wheat straw (15kg / area, equivalent to 500kg / mu) evenly between the corn rows, sprinkle the straw-degrading bacteria agent (powder) evenly on the straw, and then dissolve 1000 grams of urea in Add 1% water retaining agent to the water, and evenly spray the straw to moisten. The water-retaining agent used in the experiment is Hanlubao No. 1, Hanbao MP3005JB, Hanbao MP3005KM, Hanbao MP3005KL, Hanbao MP3005S, Hanbao anti-transpiration agent, adh...

experiment example 2

[0068] Experimental example 2 Degradation experiment of wheat straw in situ burying microbial agent

[0069] Combination experiments of straw degrading bacterial agents include: A1: Bacillus Weidenstetten C15 + Bacillus Weidenstetten C17; A2: Bacillus Weidenstetten C26 + Bacillus Weidenstetten C29; A3: Weidenstetten Bacillus C29 Bacillus Stetten JLC43+Bacillus licheniformis JLC38; CK1 (without urea); CK2 (with urea).

[0070] The experiment was carried out under field conditions, and the area of ​​each plot was 20m 2 . When the corn grows to 30-40cm, cover the wheat straw (15kg / area) evenly between the corn rows, sprinkle the straw degrading powdery bacterial agent evenly on the straw, dissolve urea (1kg / area) in water, and spray the straw evenly to moisten , Do not use water-retaining agent, turn the straw into the soil surface at a depth of 5-10cm.

[0071] (1) Influence of straw degrading bacteria agent on the degradation effect of overturned straw cellulose

[0072] Th...

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Abstract

The invention provides a microbial bacterial agent for efficiently degrading crop straws. The microbial bacterial agent comprises two strains, namely bacillus licheniformis JLC38, bacillus weihenstephanensis JLC43, and the like. The bacterial agent has the capability of efficiently degrading the crop straws under the conditions of field in-situ mulching and ploughing for field returning. The bacterial agent is suitable for in-situ quick degradation and returning of southern paddy straws and northern wheat straws and has an important effect on field in-situ returning of the crop straws. Meanwhile, the bacterial agent has obvious effects of increasing soil humus and improving soil fertility.

Description

technical field [0001] The invention belongs to the field of microbial inoculum, and in particular relates to a microbial inoculum for efficiently degrading straw containing Bacillus licheniformis JLC38 (Bacillus licheniformis) and Bacillus Weidenstetten JLC43 (Bacillus weihenstephanensis). Background technique [0002] my country is rich in crop straw resources, with a huge quantity and various types. The annual output of crop straw is 700-800 million tons. Because the straw is not easy to decompose in a short period of time, and the utilization efficiency is low, most of the straw is discarded or burned. In areas where planting is the mainstay, crop straw has become an obstacle in production. Therefore, straw burning spreads all over the vast countryside, which causes environmental pollution and serious waste of organic resources. According to statistics, the crop straw used for returning to fields and organic fertilizers in the country only accounts for about 20% of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/02C05F17/00C12R1/10C12R1/07
CPCY02W30/40
Inventor 范丙全任萍
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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