Cell cryopreserving liquid as well as preparation method and application thereof

Abstract

The invention provides a cell cryopreserving liquid as well as a preparation method and application thereof. The cell cryopreserving liquid comprises 5-20% (v / v) of cell culture solution, 65-80% (v / v) of calf serum, 10% (v / v) of dimethyl sulfoxide, 1-3% (v / v) of 3% (w / v) glutamine solution and 2-4% (v / v) of 7.5% (w / v) sodium bicarbonate solution. The cell freezing preserving liquid provided by the invention is used for culturing the cell in a cryopreserving way, thus the survival rate of the cryopreserved cells after being recovered is increased, the cell growing quality is assured and the cell proliferation time is reduced.

Description

technical field [0001] The invention relates to the technical field of tissue cell culture, in particular to a cell cryopreservation solution and its preparation method and application. Background technique [0002] Cell cryopreservation solution is a necessary solution for cell cryopreservation. For a long time, the conventional cryopreservation solution formula introduced by a large number of domestic and foreign biological cell academic books and the volume percentage of each component of the cryopreservation solution we have used for a long time are: cell Culture medium 70-80%, bovine serum 10-30%, dimethyl sulfoxide 10%. Conventionally used culture media such as MEM, M199, Eagle and DMEM have been commercialized in the market. [0003] The function of the cryopreservation solution is to disperse the cells evenly in the solution, give the cells an evenly distributed environment and space, and at the same time supply the nutrients necessary for the metabolism of the cell...

AI Technical Summary

Problems solved by technology

[0004] A large number of actual biological cytology experiments and a large number of statistical data show that the survival rate of cells frozen in this freezing solution is generally 50-70% after recovery, and the survival rate is relatively low.