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Chemiluminescent substrate liquid applied to detection system taking horseradish peroxidase (HRP) as enzymatic reaction

A luminescent substrate liquid, chemiluminescence technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, biological testing, etc., can solve the problems of instability, short validity period of luminescent substrate, high background, etc. Achieve the effect of increasing sensitivity, prolonging the reaction platform period, and quick operation

Active Publication Date: 2012-12-26
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing luminescent substrates have short validity period, high background and instability

Method used

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  • Chemiluminescent substrate liquid applied to detection system taking horseradish peroxidase (HRP) as enzymatic reaction
  • Chemiluminescent substrate liquid applied to detection system taking horseradish peroxidase (HRP) as enzymatic reaction
  • Chemiluminescent substrate liquid applied to detection system taking horseradish peroxidase (HRP) as enzymatic reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of buffer solution: prepare 0.05M borax solution and 0.2M boric acid solution respectively, then measure borax solution and boric acid solution according to the volume ratio of 11:9, and mix them to form 0.2M borate buffer solution with pH8.6. Stand at room temperature for later use.

[0038] Preparation of Luminescent Substrate Solution A: Weigh 0.3g of p-iodophenol, 0.03g of glycine, and 0.01g of bovine serum albumin, among which p-iodophenol is first dissolved in 1ml of absolute ethanol, and then the above substances are added to 100ml of 0.2M In borate buffer solution; weigh 0.01g of luminol, dissolve it in 5ml of pure water, then add it to the above buffer solution, mix well to obtain luminescent substrate solution A, and store at 4°C.

[0039] Preparation of luminescent substrate solution B: Weigh 0.02g of sodium benzoate, add it to 100ml of 0.2M borate buffer solution, then directly add 0.01g of carbamide peroxide, dissolve and mix well to obtain lumi...

Embodiment 2

[0041] Preparation of buffer solution: prepare 0.05M borax solution and 0.2M boric acid solution respectively, then measure borax solution and boric acid solution according to the volume ratio of 11:9, and mix them to form 0.2M borate buffer solution with pH8.6. Stand at room temperature for later use.

[0042] Preparation of Luminescent Substrate Solution A: Weigh 0.28g of p-iodophenol, 0.02g of glycine, and 0.008g of bovine serum albumin, among which p-iodophenol is first dissolved in 1ml of absolute ethanol, and then the above substances are added to 100ml of 0.2M In borate buffer solution; weigh 0.008g of luminol, dissolve it in 5ml of pure water, then add it to the above buffer solution, mix well to obtain luminescent substrate solution A, and store at 4°C.

[0043] Preparation of luminescent substrate solution B: Weigh 0.01g of sodium benzoate, add it to 100ml of 0.2M borate buffer solution, then directly add 0.008g of carbamide peroxide, dissolve and mix well to obtain ...

Embodiment 3

[0045] Preparation of buffer solution: prepare 0.05M borax solution and 0.2M boric acid solution respectively, then measure borax solution and boric acid solution according to the volume ratio of 11:9, and mix them to form 0.2M borate buffer solution with pH8.6. Stand at room temperature for later use.

[0046] Preparation of Luminescent Substrate Solution A: Weigh 0.32g of p-iodophenol, 0.04g of glycine, and 0.012g of bovine serum albumin, among which p-iodophenol is first dissolved in 1ml of absolute ethanol, and then the above substances are added to 100ml of 0.2M In borate buffer solution; weigh 0.012g of luminol, dissolve it in 5ml of pure water, then add it to the above buffer solution, mix well to obtain luminescent substrate solution A, and store at 4°C.

[0047]Preparation of luminescent substrate solution B: Weigh 0.03g of sodium benzoate, add it to 100ml of 0.2M borate buffer solution, then directly add 0.012g of carbamide peroxide, dissolve and mix well to obtain l...

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Abstract

The invention discloses chemiluminescent substrate liquid, which is mainly composed of radiant agent luminol, reinforcing agent p-Iodophenol, oxidant urea peroxide, and is applied to a detection system taking HRP as enzymatic reaction. The chemiluminescent substrate liquid has the characteristics of short reaction time, long detection platform period, good stability and the like, and can enhance the sensitivity of a kit, broaden linear range and prolong the period of validity of the kit.

Description

technical field [0001] The invention relates to the field of enzyme-linked immunoassay, and can be applied to a detection system in which HRP is an enzymatic reaction, such as an immunological detection system, a fluorescence detection system, and the like. Background technique [0002] Chemiluminescent immunoassay technology has been widely promoted in immunoassay, and nearly dozens of varieties are widely used, ranging from common clinical test varieties to various tumor marker screening, cytokines, drugs, etc., not only In clinical testing, it has shown broad application prospects in pharmacology, environmental science, food safety and preventive medicine. At the same time, the simplicity, speed, and sensitivity of chemiluminescence immunoassay technology make it of great value in the general survey, diagnosis, and efficacy assessment of diseases at the grassroots level. [0003] In chemiluminescent immunoassays, the amount of enzyme conjugates bound in the microwell pla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/52
Inventor 谢晶徐晶那鑫
Owner BIOSINO BIO TECH & SCI
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