Nucleic acid detection kit for 12+2 high-risk human papilloma virus (HPV)

Abstract

The invention discloses a nucleic acid detection kit for 12+2 high-risk human papilloma virus (HPV), comprising: (1) probes as shown in SEQ ID No:1-12 and primers as shown in SEQ ID No: 13-36 of a nucleotide sequence which is only complementary with the DNAs of 12 high-risk HPV; (2) a probe as shown in SEQ ID No:37 and primers as shown in SEQ ID No: 38-39 of a nucleotide sequence which is only complementary with the DNA of high-risk HPV16; (3) a probe as shown in SEQ ID No:40 and primers as shown in SEQ ID No: 41-42 of a nucleotide sequence which is only complementary with the DNA of high-risk HPV 18; and (4) a probe as shown in SEQ ID No: 43 and primers as shown in SEQ ID No: 44-45 of a nucleotide sequence which is only complementary with the DNA of an intracellular beta-globin gene, wherein each group of probes respectively mark different dyes with the maximum emission wavelengths of 518nm, 538-553nm, 574-575nm, 607-615nm, 640nm, 666-667nm and 690nm. The kit disclosed by the invention can furthest lighten huge cost brought by HPV gene detection in China, and the health of people is protected.

Description

technical field [0001] The invention relates to the field of genes, in particular to a 12+2 high-risk human papillomavirus nucleic acid detection kit. Background technique [0002] Human papillomavirus (human papillomavirus, HPV) has been concerned by people since it was found to be related to cervical cancer in the late 1970s and early 1980s. A large number of research results show that HPV plays the most important role in abnormal cervical cells and pathology of cervical cancer. Currently, more than 100 HPV genotypes have been identified, about 40 of which are associated with reproductive tract infections. The high-risk type is mainly related to the occurrence of cervical intraepithelial neoplasia (cervical intraepithelial neoplasia, CIN) II, III and cervical cancer. The low-risk type is generally not associated with malignant lesions and mainly causes genital warts and low-grade CIN (CINI). [0003] Cervical cancer is one of the common gynecological malignancies, and i...

AI Technical Summary

Problems solved by technology

However, if it is used for large-scale population screening, the cost is high, and the operation requirements are strict, complicated and time-consuming

[0008] As for the 13 high-risk detection kits of the American HC2 capture hybridization method and the domestic "13 high-risk HPV nucleic acid amplification fluorescence detection kits" have the advantages of simple operation and high degree of automation, but they cannot distinguish specific HPV types and cannot identify HPV16 and HPV18

Therefore, no matter as a clinical or screening, there are obvious defects and deficiencies.

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