Gene detection chip for lung-cancer-risk-related cytochrome P450 of smokers

Abstract

The invention provides a gene detection chip for a lung-cancer-risk-related cytochrome P450 of smokers. The gene detection chip comprises a solid-phase support and oligonucleotide probes sequentially fixed on the solid-phase support, wherein each oligonucleotide probe comprises 14-20 continuous nucleotides at mutant sites in sequences or complementary sequences, including CYP1A13798T>C, CYP1A12454A>G, CYP1A21545T>C, CYP2A6479T>A, CYP2A61436G>T, CYP2A133375C>T, CYP2E1-1053C>T. The genotypes of CYP1A1, CYP1A2, CYP2A6, CYP2A13 and CYP2E1 are detected by using a gene chip, so that the chip detection sensitivity is more than 96 percent, the specificity rate is more than 98 percent, and the characteristics of easiness for operation, accurate result and the like of gene chip detection can be brought into play. The gene detection chip plays an important realistic role in research and development units and clinical relevant applications.

Description

technical field [0001] The present invention relates to microorganisms or enzymes, belonging to a gene detection chip for gene analysis and detection products, specifically a kind of lung cancer risk-related cells of smoking population suitable for detecting the genotypes of cytochrome P450 isozymes CYP1A1, CYP1A2, CYP2A6, CYP2A13 and CYP2E1 A pigment P450 gene detection chip and a method for detecting the genotypes of CYP1A1, CYP1A2, CYP2A6, CYP2A13 and CYP2E1. technical background [0002] Cytochrome P450 isozymes are the hemoglobin superfamily, which are terminal oxidases of a mixed-function oxidase system on the endoplasmic reticulum membrane. These are called isoenzymes because of their spectrophotometric absorption peaks around 450 nm when bound to and reduced by carbon monoxide. It is now clearly believed that human cytochrome P450 is involved in the metabolism of exogenous substances (such as drugs, alcohol, antioxidants, environmental pollutants and toxic substance...

AI Technical Summary

Problems solved by technology

However, the current methods for determining CYP1A1, CYP1A2, CYP2A6, CYP2A13, and CYP2E1 genotypes mainly use methods such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), manual or automatic sequencing, and sequence-specific primer PCR. , these methods are not only cumbersome to operate, but also have a long detection cycle, and there are many factors that affect the detection results, are difficult to control, and are difficult to meet the requirements of practical applications. genotype detection

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