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Propagation method of stenoloma chusana spores

A technology of black fern spores and spores, which is applied in the breeding field that can rapidly multiply black fern spores, can solve the problems of low reproduction coefficient, difficulty in commercial production, slow speed, etc., achieves large reproduction coefficient, simple and practical operation, and prevents bacterial contamination. Effect

Inactive Publication Date: 2011-08-10
李红 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The propagation of C. sylvestris can be carried out by branching and spore propagation. The propagation speed of sclerotia is slow and the reproduction coefficient is low, so it is difficult to apply to commercial production. At present, there are few studies on the conventional spore propagation of C.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] When the spores of the black fern are about 30% mature, cut off the leaves with mature spores and put them on a clean white paper to dry in the shade. After the spores are completely scattered, according to the quality of the spores and sporangia, 0.03 g of the spores with higher purity are manually selected, wrapped with filter paper, soaked in 5% NaClO solution for 5 minutes, and then quickly rinsed with sterile water.

[0014] Sand, perlite and peat are mixed in a volume ratio of 1:1:1 to obtain a seeding substrate with a pH value of 6.0 to 7.0, and the sterilized black fern spores are sown on the prepared substrate. The culture temperature is 23-33°C, the light intensity is 3000-5000lx, the light time is 9-12h / d, and the mixing rate is 65-80%. After 8 days of sowing, observe under a magnifying glass that the spores germinate successively. After 15 days, the spores can be seen to germinate with the naked eye. After 25 days, the gametophytes develop into sheets. After...

Embodiment 2

[0017] When the spores of the black fern are about 50% mature, cut off the leaves with mature spores and put them on a clean white paper to dry in the shade. After the spores are completely scattered, according to the quality of the sporangia, manually select 0.03 g of spores with higher purity water, wrap them with filter paper, put them into 5% NaClO solution and soak them for 5 minutes, and then quickly rinse them with sterile distilled water.

[0018] Sand, perlite and peat are mixed in a volume ratio of 1:1:1 to obtain a seeding substrate with a pH value of 6.0 to 7.0, and the sterilized Sarcodon spores are sown on the prepared substrate. The culture temperature is 23-33°C, the light intensity is 3000-5000lx, the light time is 9-12h / d, and the humidity is 65-80%. After 8 days of sowing, observe under a magnifying glass that the spores germinate successively. After 15 days, the spores can be seen to germinate with the naked eye. After 25 days, the gametophyte develops into...

Embodiment 3

[0021] When the spores of the black fern are about 30% mature, cut off the leaves with mature spores and put them on a clean white paper to dry in the shade. After the spores are completely scattered, according to the quality of the spores and sporangia, 0.03 g of spores with higher purity are manually selected, wrapped with filter paper, soaked in 10% NaClO solution for 5 minutes, and then quickly rinsed with sterile distilled water.

[0022] Sand, perlite and peat are mixed in a volume ratio of 1:1:1 to obtain a seeding substrate with a pH value of 6.0 to 7.0, and the sterilized black fern spores are sown on the prepared substrate. The culture temperature is 23-33°C, the light intensity is 3000-5000lx, the light time is 9-12h / d, and the humidity is 65-80%. 8 days after sowing, observe under a magnifying glass, the spores germinated successively. The spores germinated visible to the naked eye after 20 days. After 45 days, the gametophytes developed into flakes. After 70 days,...

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PUM

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Abstract

The invention relates to a propagation method of stenoloma chusana spores, which comprises the following steps of: mature stenoloma chusana spore collecting, ground substance disinfecting, spore sterilizing, spore germinating, zygote forming, gametophyte growing, antiphyte growing, seedling transplanting and the like. The method can be used for fast propagating the stenoloma chusana, is simple in the preparation of the cultivation ground substance, is simple and convenient in operation method, is high in operability, and has the advantages of being small in investment and high in yield, so that the transplanting survival rate of the spore seedlings reaches more than 85%.

Description

technical field [0001] The invention relates to a spore propagation method of Sphenomeris chinenses, in particular to a propagation method capable of rapidly propagating Sphenomeris chinenses spores. Background technique [0002] Sphenomeris is a plant of the genus Sphenomeris in the family Phytofernaceae. It is a small and medium-sized terrestrial fern widely distributed in tropical and subtropical regions. It is born in low mountain forests and roadsides, and is light-loving and drought-tolerant. The leaf color of black fern is emerald green or yellow-green, the leaves are thinner, the leaf shape is longer, the shape is elegant, the posture is beautiful, it has high ornamental value, and it is more drought-tolerant. It can be planted alone in places with better light in the green space, can be planted in sheets or clusters, can also be planted with other plants for landscaping, and can also be used for potted ornamentals. It is a relatively good ornamental fern. The whole...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G31/00A01C1/00
Inventor 李红孙起梦刘兴剑
Owner 李红
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