Cadmium sulfide quantum dot biosynthesis method, prepared cadmium sulfide quantum dots and application of cadmium sulfide quantum dots

Abstract

The invention discloses a cadmium sulfide quantum dot biosynthesis method, prepared cadmium sulfide quantum dots and application of the cadmium sulfide quantum dots. The biosynthesis method comprisesthe following steps of by taking sulfur-containing amino acid as an S source, water-soluble cadmium salt as a Cd source, smCSE as a biocatalyst and protease as a reaction terminator, performing synthesis to prepare the cadmium sulfide quantum dots, and applying the cadmium sulfide quantum dots prepared in the step to the field of cell labeling. Compared with the prior art, the synthesis method provided by the invention is environmentally friendly and is strong in controllability; and the cadmium sulfide quantum dots prepared by the method is regular in shape, high in yield and good in stability and can be used for cell labeling or chemical detection.

Description

technical field [0001] The invention relates to the field of nanomaterial preparation, in particular to a biosynthesis method of cadmium sulfide quantum dots, the prepared cadmium sulfide quantum dots and applications thereof. Background technique [0002] Quantum Dots (QDs) are semiconductor nanocrystals, which have some unique fluorescent properties due to the size effect and quantum confinement effect of nanomaterials. [1-3] , such as controllable fluorescence emission wavelength, narrow and symmetrical emission peak, wide excitation wavelength range, high quantum efficiency, and good photostability, etc. Therefore, quantum dots have attracted extensive attention as an ideal fluorescent reporter group for immunoassays. However, the current synthesis method mainly adopts the traditional chemical synthesis method, which is not green and environmentally friendly. [0003] Chinese invention patent CN102965406B discloses a biosynthesis method of cadmium sulfide quantum dots. ...

AI Technical Summary

Problems solved by technology

However, in the literature, the reaction cannot be terminated at any time, and ultrapure water dialysis is required to obtain quantum dots. During the reaction, the functions of smCSE mainly include two aspects: synthesizing cadmium sulfide and mediating the growth of quantum dots. Under water dialysis, the growth of quantum dots cannot be terminated, and the solution will eventually become a fluorescence-quenched cadmium sulfide solution, and the reaction cannot be terminated immediately under ultrapure water dialysis, so this method cannot be used in a high-concentration system (because the reaction is too high). fast, fluorescence quenching occurs immediately) cannot reasonably control the synthesis size of quantum dots, and the quality of the synthesized quantum dots reported after dialysis is not good (low quantum yield and irregular shape)

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