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Hyaluronate gel particle and preparation method thereof

A technology of hyaluronate and gel particles, applied in medical science, surgery, etc., can solve the problems of short decomposition period, complex preparation method, fast degradation and absorption, etc., to reduce production and operation costs, and to achieve high operational feasibility , the effect of easy injection into the body

Inactive Publication Date: 2011-07-13
深圳市资福药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there are many gel cross-linking methods, such as the gel preparation method disclosed in CN159044A, but the raw materials used are of low purity and need to be deproteinized first, which makes the preparation method more complicated and the cycle longer; And the cross-linking method that CN101264348 used is to join hyaluronic acid in the sodium hyaluronate solution, cross-link by cross-linking agent again, but its cross-linking effect is not very ideal, and decomposition period is short; The disclosed method of CN101502677 gel Sieve into particles under full expansion, then use buffer to shrink them, and finally filter or centrifuge to collect particles, which increases the difficulty in the operation process. The separation effect of gel particles and buffer by centrifugation is not ideal, and the coagulation Glue particles are too small to degrade and absorb too quickly in the body

Method used

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  • Hyaluronate gel particle and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Add 1.0 g sodium hyaluronate dry powder (molecular weight 1.8 million Daltons, purchased from Shandong Freda) to 10 ml of 0.8% sodium hydroxide solution, stir until it becomes transparent and fully dissolve, and then add 80 μl of sodium hyaluronate Glycol diglycidyl ether (CA: 2425-79-8), stirred evenly, and reacted at a constant temperature of 30°C for 7 hours to obtain a transparent gel block, which was added to 500ml of deionized water, kept at a constant temperature of 70°C for 6 hours, purified Change the water once during the swelling process, filter, and wash the resulting gel piece with 500ml PBS damping fluid (the osmotic pressure is 350, and every 1000ml contains NaH 2 PO 4 2H 2 O 0.099mg, NaH 2 PO 4 2H 2 (01.123g, NaCl 10.45g) static dialysis for 12 hours, during which the PBS buffer solution was changed twice, filtered, and the gel piece was passed through an 80-mesh stainless steel sieve to obtain sodium hyaluronate gel particles.

Embodiment 2

[0033] Add 1.0 g sodium hyaluronate dry powder (molecular weight 1.8 million Daltons, purchased from Shandong Freda) to 10 ml of 0.8% sodium hydroxide solution, stir until it becomes transparent and fully dissolve, and then add 80 μl of sodium hyaluronate Diol diglycidyl ether (CA: 2425-79-8), stir well, react at 40°C for 4 hours to obtain a transparent gel block, add it to 500ml deionized water, keep at 70°C for 6 hours, purify and swell Change water once in the process, filter, with 500ml PBS damping fluid (osmotic pressure is 350, every 1000ml contains NaH 2 PO 4 2H 2 O 0.099mg, NaH 2 PO 4 2H 2 O 1.123g, NaCl 10.45g) static dialysis for 12 hours, during which the PBS buffer solution was replaced twice, filtered, and the gel piece was passed through an 80-mesh stainless steel sieve to obtain sodium hyaluronate gel particles.

Embodiment 3

[0035] Add 1.0 g sodium hyaluronate dry powder (molecular weight 1.8 million Daltons, purchased from Shandong Freda) to 10 ml of 0.8% sodium hydroxide solution, stir until it becomes transparent and fully dissolve, and then add 80 μl of sodium hyaluronate Diol diglycidyl ether (CA: 2425-79-8), stirred evenly, and reacted at a constant temperature of 50°C for 4 hours to obtain a transparent gel block, which was added to 500ml of deionized water, kept at a constant temperature of 70°C for 6 hours, and purified Change the water once during the swelling process, filter, and wash the resulting gel piece with 500ml PBS damping fluid (the osmotic pressure is 350, and every 1000ml contains NaH 2 PO 4 2H 2 O 0.099mg, NaH 2 PO 4 2H 2 (01.123g, NaCl 10.45g) static dialysis for 12 hours, during which the PBS buffer solution was changed twice, filtered, and the gel piece was passed through an 80-mesh stainless steel sieve to obtain sodium hyaluronate gel particles.

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Abstract

The invention relates to a hyaluronate gel particle and a preparation method thereof. The preparation method of the hyaluronate gel particle comprises the following steps: crosslinking hyaluronate and a crosslinking agent, forming a gel after absorbing water to swell, equilibrizing and dialyzing by using a physiological equilibrium liquid, and finally screening to obtain the hyaluronate gel particle. The hyaluronate gel particle prepared by the method provided by the invention has better crosslinking effect, and the viscosity, particle size and stability of the hyaluronate gel particle are very suitable for beauty treatment.

Description

technical field [0001] The invention relates to a hyaluronate gel particle and a preparation method thereof. Background technique [0002] Hyaluronic acid (HA for short), also known as uronic acid. Natural hyaluronic acid is a polymer polysaccharide, which is a linear polymer polysaccharide composed of glucuronic acid-N-glucosamine acetate as a disaccharide molecular unit, with an average molecular weight between 100,000 and 10 million Daltons. present in the intercellular space. Hyaluronic acid can be extracted from the connective tissues of vertebrates, such as cockscombs, eyeballs, umbilical cords, and cartilages. In the past, the source of hyaluronic acid was mostly extracted from animals, but now it is artificially synthesized, which eliminates problems such as allergies and infections. [0003] Hyaluronic acid was originally only used for moisturizing. Because of its high viscoelasticity, good biocompatibility and non-immunogenicity, it will not cause allogeneic rej...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08J3/24C08J3/12A61L27/20A61L31/04
Inventor 郝宏山
Owner 深圳市资福药业有限公司
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