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Method for culturing aseptic seedlings of plants

A cultivation method and technology of sterile seedlings, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as adverse effects on seed germination, interference with plant growth, and difficult washing and removal, so as to improve seed germination rate, Good growth of stems and leaves, and the effect of avoiding contamination by miscellaneous bacteria

Inactive Publication Date: 2011-07-13
SOUTHEAST UNIV
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Problems solved by technology

If using a strong disinfectant such as 0.1% HgCl 2 , or 2% sodium hypochlorite solution as reported in the literature, the disinfectant will penetrate through the seed coat, and it is not easy to wash and remove. The residual disinfectant will have a negative impact on seed germination, not only greatly reducing the emergence rate, but also The residual disinfectant may interfere with the follow-up The growth of plants is not conducive to the study of the response mechanism of Arabidopsis to environmental factors (such as: heavy metal ions, high salt, etc.)

Method used

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  • Method for culturing aseptic seedlings of plants

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Embodiment Construction

[0030] In order to solve the problems of the technologies described above, the specific implementation method that the present invention adopts is:

[0031] a. The preparation of the nutrient matrix of seed germination and seedling growth: adopt agar powder for tissue culture, add MS (MS is the abbreviation of MS medium inventor Murashige and Skoog, the same below) macronutrient mother liquor, MS micronutrient mother liquor and MS iron salt mother solution, adjust pH to 5.8, autoclave: 121°C, 20 minutes, nutrient agar solidifies and set aside;

[0032] b. Plant seed surface disinfection method: plant seeds are placed in a microcentrifuge tube, sterilized with 75%, 95% ethanol solution and absolute ethanol successively, washed with sterile water, and then spread on the nutrient matrix;

[0033] -3-

[0034] c. Germination of plant seeds: After the nutrient matrix for seed germination is prepared, it is divided into sterile jars (specification: 250mL), each bottle is filled wit...

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Abstract

The invention relates to a technology for culturing aseptic seedlings of plants and application thereof, particularly suitable for seedlet plants such as model plant arabidopsis thaliana, medicinal plant artemisia annua, fruit and vegetable tomato, pepper and the like. The method gets rid of the disturbances of other microbes in the environment and is particularly suitable for searching mutual action between the plants and the microbe. The method comprises steps of: preparing nutritious substrates for seed germination and seedling growth, sterilizing the surfaces of the plant seeds, and germinating the plant seeds. The method is a convenient and efficient g method for culturing aseptic seedlings of plants and has low cost; in addition, the method is free of sterilizing agent residual, thereby ensuring the reliability and accuracy of subsequent research results.

Description

technical field [0001] The invention relates to a plant aseptic seedling cultivation technology and its application. Especially suitable for small seed plants, such as model plants Arabidopsis, medicinal plants Artemisia annua, fruits and vegetables tomatoes, peppers, etc. Because it excludes the interference of other microorganisms in the environment, it is especially suitable for studying the interaction between plants and microorganisms. Background technique [0002] Since the 1970s and 1980s, with the rise of molecular biology, biologists began to look for a model plant suitable for fine research at the level of genetics and molecular biology, the flowering plant Arabidopsis thaliana ( Arabidopsis thaliana) has been found to be well suited for studies in plant genetics, developmental biology, and molecular biology. In recent years, many important discoveries in the field of plant-related research, such as root growth, flowering, hormone regulation, and plant response m...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 林岚
Owner SOUTHEAST UNIV
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