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Medicinal composition having immunity-regulating function

A technology for medicines and extracts, applied in the field of Bupleurum edulis extracts, can solve problems such as researches or literatures on the regulation of immune function of Bupleurum edulis that have come out yet.

Active Publication Date: 2013-08-21
IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Bupleurum is currently the subject of active development in crude drugs for the treatment of hepatitis, however, there is no research or literature on the application of Bupleurum in regulating immune function

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 screening plant material

[0048] Select the roots of various Bupleurum plants and grind them into plant powder.

[0049] Get above-mentioned plant powder 0.5g, after shaking overnight at room temperature with 25ml of 95wt% ethanol, redissolve into 2ml with 95wt% alcohol after drying and concentrating, carry out high performance liquid chromatography (HPLC) analysis, HPLC analysis condition is as follows:

[0050] Column: SymmetryShield TM (Waters), RP 18, 5μm, 250×4.6mmlD

[0051] Mobile phase: A: H 2 O, B: Acetonitrile, C: Methanol

[0052] Detection: UV / λ=237nm.

[0053] Among these Bupleurum extracts, select and standard dibenzylbutyrolactone type lignan (kaerophyllin) ( figure 1 Middle (a) line) has the same peak ( figure 1 The peak in the frame) Bupleurum, figure 1 The (b) line represents (b) Bupleurum (i.e., sequence identification number 1); (c) line represents Altai Bupleurum (i.e., the sequence identification number 2 Bupleurum); (d) line ...

Embodiment 2

[0054] Embodiment 2 extracts active ingredient

[0055] Grind the Altai Bupleurum krylovianum plant selected in Example 1, take 2kg of powder and add it to 16L of 95wt% ethanol aqueous solution, after 5 hours at room temperature, dry and concentrate to obtain an extract with an extraction rate of 6.7±0.1%. Perform high performance liquid chromatography (HPLC) analysis, such as figure 2 As shown, there is a peak between 30-40 minutes.

[0056] HPLC analysis conditions:

[0057] Column: SymmetryShield TM (Waters), RP 18, 5μm, 250×4.6mmlD

[0058] Mobile phase: A: H 2 O, B: Acetonitrile, C: Methanol

[0059]Detection: UV / λ=237nm

Embodiment 3

[0060] Example 3 Identification of active ingredients

[0061] 18.5 kg of Bupleurum krylovianum screened in Example 1 was added to 25 L of methanol, and after 4 hours at room temperature, it was concentrated and dried to obtain 18.5 kg of a residue. Take this residue 500mg, carry out column chromatography, with SiO 2 As the stationary phase, use hexane, ethyl acetate, methanol (6 / 4 / 1-3 / 2 / 1-0 / 0 / 1 to adjust different volume ratios) as the mobile phase, and after washing the column with a polar gradient, They were collected separately and analyzed by thin layer chromatography (TLC) to obtain 12 fractions. These 12 parts are carried out following HLPC analysis, and 12 parts are dissolved in methanol respectively, and concentration is adjusted to 1mg / ml, selects the part that has identical peak with standard product dibenzylbutyrolactone type lignan (kaerophyllin), will This part was subjected to the above-mentioned column chromatography again to separate the extract with higher ...

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Abstract

The invention relates to a medicinal composition having immunity-regulating function. The medicinal composition comprises bupleurum extract, and the bupleurum extract is prepared by the following steps of: adding bupleurum powder into a solvent, separating extract from the solvent, wherein the bupleurum comprises: bupleurum krylovianum, bupleurumlongicaule, bupleurum longiradiatum, bupleurupusillum, bupleurum salicifolium, bupleurumscorzonerifolium, bupleurum smithii, sequence identification No.1 bupleurum, sequence identification No.2 bupleurum, sequence identification No.3 bupleurum, sequence identification No.4 bupleurum, sequence identification No.5 bupleurum, sequence identification No.6 bupleurum or bupleurum of which the difference value is within 1 percent with the sequences, or acombination of the raw materials.

Description

technical field [0001] The invention relates to a pharmaceutical composition with immune regulation function, in particular to Bupleurum bupleuri extract with immune regulation function. Background technique [0002] In recent years, there have been many studies on the physiological mechanism of diseases caused by the immune system. Many studies point to the relationship between the excessive expression of tumor necrosis factor (TNF-α) and immune inflammatory diseases, such as rheumatoid arthritis, Crohn's disease, psoriatic arthritis or ankylosing arthritis etc. [0003] TNF-[alpha] acts like a biological hormone, such as regulating cell recruitment, cell proliferation, cell death, or immunomodulation. When TNF-α is in low concentration in the tissue, it can amplify the defense mechanism of the host against foreign infection. At high concentrations, TNF-alpha can cause excessive inflammatory responses that cause organ damage. Therefore, Brennan et al. proposed that remov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/233A61P37/02A61P37/06A61P31/04A61P29/00A61P19/02A61P19/04A61P19/10A61P17/00A61P1/00A61P35/00A61P21/00A61P25/28A61P25/02A61P1/02A61P3/10A61P27/02A61P27/06A61P13/12A61P11/00A61P11/02A61P11/06A61P1/16A61P9/10A61K125/00
Inventor 石英珠李连滋蔡必胜罗吉孟童天送许振霖黄国魁蔡莹霏李怡静梁惠如颜瑞宏李承榆
Owner IND TECH RES INST
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