Cultivation method of lucilia sericata larva and disinfection method thereof

Abstract

The invention provides a cultivation method of lucilia sericata larva and a disinfection method thereof, in particular to the disinfection to the egg and the larva in the process of cultivating. The disinfection method of the egg comprises the steps of: dipping the egg masses into 0.5-1% sodium sulfite solution, shaking at 3-5 minutes, so that the cohesive egg masses are separated from one another, and dipping the egg into 5-6% sodium hypochlorite solution to disinfect at 3-5 minutes after filtering; and the disinfection method of the larva comprises the step of disinfecting the larva by 0.3-0.5% iodophor solution and 0.1% bromo-geramine solution at two steps. The methods less influence the vitality of the fly egg, the death rate is 0%, the disinfection action to the larva is fast and durable, the toxicity is low, and the methods are less influenced by the organic matter; and the effective passage of the larva can be kept, the vitality of the disinfected lucilia sericata larva is kept, and the methods are simple to operate, easy to grasp, and suitable for popularization and application on a large scale.

Description

technical field [0001] The invention relates to a method for cultivating insects and disinfecting live insects, in particular to a method for cultivating and disinfecting Lucilia sericata larvae. Background technique [0002] The insect larvae used clinically to treat infected wounds are mainly the larvae of Lucilia sericata. Lucilia sericata, also known as the blowfly, is distributed worldwide. Belongs to Diptera, adult fly body length 5mm ~ 10mm. The body color is green with metallic luster, the cheeks are silvery white, the mane on the middle thorax is developed, and the axillary flap is hairless. There are more than 6 small hairs in the back of the shoulder mane, at the narrowest part of the forehead. The length of the stem of the fifth web is greater than 1 / 2 of the length of the lateral leaves. Anal lobe posteriorly tapering apex to terminal. Lucilia sericata goes through four stages in its life: eggs, larvae (maggots), pupae, and adults (flies). Its larval stage ...

AI Technical Summary

Problems solved by technology

Lucilia sericata goes through four stages in its life: eggs, larvae (maggots), pupae, and adults (flies). Its larval stage can be used as a medical insect, has scavenging properties, and has a strong ability to reproduce and grow in the natural environment, but it is artificially raised indoors. , There is no mature method for reproduction and disinfection

At present, there is a combination of sulfurous acid and formaldehyde to disinfect the eggs, and then hatch them in a sterile environment, raise them with sterilized food, and apply them clinically, so as to avoid secondary infection, but use sulfurous acid and formaldehyde to disinfect the eggs It will lead to an increase in the probability of egg death, which is not conducive to stable passage

There is also application of 3% creol soap solution to sterilize the eggs, and aseptic rearing and subculture, but there is still a 20-30% mortality rate

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