Homocysteine measuring method and reagent

Abstract

The invention discloses a method for measuring homocysteine concentration. The method comprises the following steps of: placing a sample to be measured into an enzymatic circular reaction system, performing reaction shown as formulas 1 to 3, measuring and calculating the production rate of ammonia produced in the enzymatic circular reaction system so as to obtain the content of homocysteine in the sample to be measured; and the enzymatic circular reaction system comprises methionine synthase, methionine gama-lyase, O-acetyl homoserine aminocarboxypropyl transferase, 5-methyltetrahydrofolate or salt of the 5-methyltetrahydrofolate, and O-acetyl-L-homoserine. The invention also discloses a corresponding measuring reagent. The invention provides a novel homocysteine enzymological measuring method and a measuring reagent. The method does not need any special instrument, is easy and convenient to operate, has high sensitivity, is low in cost, can realize the clinical, quick and high-flow sample detection, ensures that the homocysteine possibly becomes the clinical conventional detection item, and has high scientific and economic value. The formulas 1 to 3 are shown in the specifications.

Description

A kind of homocysteine ​​determination method and reagent thereof technical field The invention belongs to the technical field of medical testing and determination, in particular to a homocysteine ​​determination reagent. Background technique Homocysteine ​​is a sulfur-containing amino acid produced by demethylation of methionine in cells. Recent studies have shown that homocysteine ​​damages vascular endothelial cells through the production of superoxide and peroxides, changes the function of coagulation factors, increases the tendency of thrombosis, promotes atherosclerosis and thrombosis, and increases the incidence of cardiovascular disease and thrombus formation. Mortality increases, so the determination of the concentration of homocysteine ​​in blood is of clinical significance. When homocysteine ​​accumulates in cells and enters the blood circulation, most of it exists in the oxidized form and binds to proteins, and the reduced form of homocysteine ​​accounts for o...

AI Technical Summary

Problems solved by technology

The technical problem to be solved by the present invention is on the one hand to overcome the defects that the existing homocysteine ​​determination method requires special instruments, complex operation, long time consumption, and cannot perform automatic analysis of large flow rate; on the other hand, it is to further improve the present invention It has the sensitivity of a fully automatic analytical reagent, thereby improving the quality of the determination, and provides a homocysteine ​​determination reagent

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